Analysis of post-translational modification of mycobacterial proteins using a cassette expression system

被引：50

作者：

Herrmann, JL ^{[1
]}

Delahay, R ^{[1
]}

Gallagher, A ^{[1
]}

Robertson, B ^{[1
]}

Young, D ^{[1
]}

机构：

[1] Imperial Coll Sch Med, Dept Infect Dis & Microbiol, London W2 1PG, England

来源：

FEBS LETTERS | 2000年 / 473卷 / 03期

基金：

英国惠康基金;

关键词：

mycobacterium; O-glycoprotein; post-translational modification; tuberculosis;

D O I：

10.1016/S0014-5793(00)01553-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites,within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have an important influence on glycosylation. The expression system was used to screen mycobacterial sequences selected using a neural network (NetOglyc) trained on eukaryotic O-glycoproteins. Evidence of glycosylation was obtained for eight of 11 proteins tested. The results suggest that sites involved in O-glycosylation of mycobacterial and eukaryotic proteins share similar structural features. (C) 2000 Federation of European Biochemical Societies.

引用

页码：358 / 362

页数：5

共 32 条

[1] Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2 [J].

Aliprantis, AO ;

Yang, RB ;

Mark, MR ;

Suggett, S ;

Devaux, B ;

Radolf, JD ;

Klimpel, GR ;

Godowski, P ;

Zychlinsky, A .

SCIENCE, 1999, 285 (5428) :736-739

[2] EVIDENCE THAT PROTEIN ANTIGEN-B OF MYCOBACTERIUM-TUBERCULOSIS IS INVOLVED IN PHOSPHATE-METABOLISM [J].

ANDERSEN, AB ;

LJUNGQVIST, L ;

OLSEN, M .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :477-480

[3] A HIGHLY IMMUNOGENIC PUTATIVE MYCOBACTERIUM-KANSASII LIPOPROTEIN [J].

ARMOA, GRG ;

ROUSE, DA ;

NAIR, J ;

MACKALL, JC ;

MORRIS, SL .

MICROBIOLOGY-UK, 1995, 141 :2705-2712

[4] NUCLEOTIDE-SEQUENCE OF THE 19 KDA ANTIGEN GENE FROM MYCOBACTERIUM-TUBERCULOSIS [J].

ASHBRIDGE, KR ;

BOOTH, RJ ;

WATSON, JD ;

LATHIGRA, RB .

NUCLEIC ACIDS RESEARCH, 1989, 17 (03) :1249-1249

[5] HOMOLOGS OF MYCOBACTERIUM-LEPRAE 18-KILODALTON AND MYCOBACTERIUM-TUBERCULOSIS 19-KILODALTON ANTIGENS IN OTHER MYCOBACTERIA [J].

BOOTH, RJ ;

WILLIAMS, DL ;

MOUDGIL, KD ;

NOONAN, LC ;

GRANDISON, PM ;

MCKEE, JJ ;

PRESTIDGE, RL ;

WATSON, JD .

INFECTION AND IMMUNITY, 1993, 61 (04) :1509-1515

[6] Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors [J].

Brightbill, HD ;

Libraty, DH ;

Krutzik, SR ;

Yang, RB ;

Belisle, JT ;

Bleharski, JR ;

Maitland, M ;

Norgard, MV ;

Plevy, SE ;

Smale, ST ;

Brennan, PJ ;

Bloom, BR ;

Godowski, PJ ;

Modlin, RL .

SCIENCE, 1999, 285 (5428) :732-736

[7] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[8] Definition of the full extent of glycosylation of the 45-kilodalton glycoprotein of Mycobacterium tuberculosis [J].

Dobos, KM ;

Khoo, KH ;

Swiderek, KM ;

Brennan, PJ ;

Belisle, JT .

JOURNAL OF BACTERIOLOGY, 1996, 178 (09) :2498-2506

[9]

ESPITIA C, 1989, CLIN EXP IMMUNOL, V77, P378

[10] PURIFICATION AND CHARACTERIZATION OF MAJOR ANTIGENS FROM A MYCOBACTERIUM-BOVIS CULTURE FILTRATE [J].

FIFIS, T ;

COSTOPOULOS, C ;

RADFORD, AJ ;

BACIC, A ;

WOOD, PR .

INFECTION AND IMMUNITY, 1991, 59 (03) :800-807

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