Dissecting dynamin's role in clathrin-mediated endocytosis

被引:140
作者
Mettlen, Marcel [1 ]
Pucadyil, Thomas [1 ]
Ramachandran, Rajesh [1 ]
Schmid, Sandra L. [1 ]
机构
[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
基金
美国国家卫生研究院;
关键词
clathrin-mediated endocytosis; clathrin-coated vesicle; dynamin; fission; GTPase; vesicle formation; DEPENDENT CONFORMATIONAL-CHANGES; MECHANOCHEMICAL ENZYME DYNAMIN; COATED VESICLE FORMATION; GTP-BINDING PROTEINS; DOMAIN; DROSOPHILA; MEMBRANE; CELL; MICROTUBULES; CONSTRICTION;
D O I
10.1042/BST0371022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The GTPase dynamin is essential for CME (clathrin-mediated endocytosis), but its exact function and mechanism of action have been controversial. Here, we review findings that have led to the current models for dynamin function, either as a mechanochemical enzyme driving membrane fission or as a regulatory GTPase monitoring rate-limiting steps in CME. However, these models are not mutually exclusive and subsequent studies have provided evidence for both dynamin functions. Recent evidence derived from divergent in vivo and in vitro approaches suggests that dynamin plays a dual role in CME, functioning at early stages as a fidelity monitor to regulate clathrin-coated pit maturation and at later stages to directly catalyse membrane fission and clathrin-coated vesicle formation.
引用
收藏
页码:1022 / 1026
页数:5
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