Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response

被引：230

作者：

Bauman, Susanne J. ^{[1
]}

Kuehn, Meta J. ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

来源：

MICROBES AND INFECTION | 2006年 / 8卷 / 9-10期

关键词：

Pseudomonas aeruginosa; outer membrane; vesicles; cystic fibrosis; aminopeptidase; pathogenesis;

D O I：

10.1016/j.micinf.2006.05.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Considerable lung injury results from the inflammatory response to Pseudomonas aeruginosa infections in patients with cystic fibrosis (CF). The P. aeruginosa laboratory strain PAO1, an environmental isolate, and isolates from CF patients were cultured in vitro and outer membrane vesicles from those cultures were quantitated, purified, and characterized. Vesicles were produced throughout the growth phases of the culture and vesicle yield was strain-independent. Strain-dependent differences in the protein composition of vesicles were quantitated and identified. The aminopeptidase PaAP (PA2939) was highly enriched in vesicles from CF isolates. Vesicles from all strains elicited IL-8 secretion by lung epithelial cells. These results suggest that P. aeruginosa colonizing the CF lung may produce vesicles with a particular composition and that the vesicles could contribute to inflammation. (c) 2006 Elsevier SAS. All rights reserved.

引用

页码：2400 / 2408

页数：9

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