The mouse dead-end gene isoform α is necessary for germ cell and embryonic viability

被引：44

作者：

Bhattacharya, Chitralekha

Aggarwal, Sita

Zhu, Rui

Kumar, Madhu

Zhao, Ming

Meistrich, Marvin L.

Matin, Angabin ^{[1
]}

机构：

[1] Univ Texas, MD Anderson Canc Ctr, Dept Canc Genet, Houston, TX 77030 USA

[2] Univ Texas, MD Anderson Canc Ctr, Dept Expt Radiat Oncol, Houston, TX 77030 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 355卷 / 01期

关键词：

testicular germ cell tumors; dead-end; Dnd1; isoform; antibodies; Ter; STEM-CELLS; TESTICULAR TERATOMAS; TER MUTATION; MICE; DEFICIENCY;

D O I：

10.1016/j.bbrc.2007.01.138

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inactivation of the dead-end (Dnd1) gene in the Ter mouse strain results in depletion of primordial germ cells (PGCs) so that mice become sterile. However, on the 129 mouse strain background, loss of Dnd1 also increases testicular germ cell tumor incidence in parallel to PGC depletion. We report that inactivation of Dndl also affects embryonic viability in the 129 strain. Mouse Dnd1 encodes two protein isoforms, DND1-isoform alpha (DND1-alpha) and DND1-isoform beta (DND1-beta). Using isoform-specific antibodies, we determined DND1-alpha is expressed in embryos and embryonic gonads whereas DND1-beta expression is restricted to germ cells of the adult testis. Our data implicate DND1-alpha isoform. to be necessary for germ cell viability and therefore its loss in Ter mice results in PGC depletion, germ cell tumor development and partial embryonic lethality in the 129 strain. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：194 / 199

页数：6

共 24 条

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