Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system

被引：20

作者：

Mie, M ^{[1
]}

Ohgushi, H

Yanagida, Y

Haruyama, T

Kobatake, E

Aizawa, M

机构：

[1] Tokyo Inst Technol, Dept Biol Informat, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[2] Nara Med Univ, Dept Orthoped, Kashihara, Nara 634, Japan

来源：

TISSUE ENGINEERING | 2000年 / 6卷 / 01期

关键词：

D O I：

10.1089/107632700320847

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

A novel tissue engineering for bone formation has been proposed, to make osteoblast differentiation balanced by transfecting the mesenchymal stem cells with a gene encoding human bone morphogenetic protein-2 (hBMP-2) under the control of adipocyte specific lipoprotein lipase (LPL) promoter. Due to the promoter specificity, the initiation of BMP transcription is dependent on adipogenesis. For 14-day culture in the presence of ascorbic acid (asc) and beta-glycerophosphate (gly), nontransfected mouse embryonic fibroblast C3H10T1/2 (10T1/2) cells showed extensive accumulation of lipid droplets and adipocyte specific enzyme glycerol-3-phosphate dehydrogenase (G3PDH) mRNA expression, but exhibited neither BMP-2 expression, high alkaline phosphatase (ALP) activity which reflects osteoblast phenotype; On the other hand, transfected 10T1/2 cells showed hBMP-2 expression, high ALP activity and low level of G3PDH. mRNA expression accompanied with minimal lipid droplets. These results indicate that 10T1/2 cells are proved to be differentiated with maintaining coordinated balance of adipogenesis and osteogenesis, when they are transfected by the gene encoding hBMP-2 under the control of LPL promoter.

引用

页码：9 / 18

页数：10

共 33 条

[1] EXPRESSION OF HUMAN BONE MORPHOGENETIC PROTEINS-2 OR PROTEINS-4 IN MURINE MESENCHYMAL PROGENITOR C3H10T1/2 CELLS INDUCES DIFFERENTIATION INTO DISTINCT MESENCHYMAL CELL LINEAGES [J].