Nuclear Pore Complex Component MOS7/Nup88 Is Required for Innate Immunity and Nuclear Accumulation of Defense Regulators in Arabidopsis

被引:211
作者
Cheng, Yu Ti [1 ,2 ]
Germain, Hugo [1 ]
Wiermer, Marcel [1 ]
Bi, Dongling [3 ]
Xu, Fang [1 ,3 ,4 ]
Garcia, Ana V. [5 ]
Wirthmueller, Lennart [5 ]
Despres, Charles [6 ]
Parker, Jane E. [5 ]
Zhang, Yuelin [3 ]
Li, Xin [1 ,2 ,4 ]
机构
[1] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada
[2] Univ British Columbia, Grad Program Genet, Vancouver, BC V6T 1Z3, Canada
[3] Natl Inst Biol Sci, Beijing 102206, Peoples R China
[4] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada
[5] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
[6] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
SYSTEMIC ACQUIRED-RESISTANCE; NBS-LRR PROTEINS; NF-KAPPA-B; DISEASE-RESISTANCE; SIGNAL-TRANSDUCTION; SALICYLIC-ACID; NUCLEOCYTOPLASMIC TRANSPORT; MESOPHYLL PROTOPLASTS; TRANSCRIPTION FACTORS; NUCLEOPORIN NUP145P;
D O I
10.1105/tpc.108.064519
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plant immune responses depend on dynamic signaling events across the nuclear envelope through nuclear pores. Nuclear accumulation of certain resistance (R) proteins and downstream signal transducers are critical for their functions, but it is not understood how these processes are controlled. Here, we report the identification, cloning, and analysis of Arabidopsis thaliana modifier of snc1,7 (mos7-1), a partial loss-of-function mutation that suppresses immune responses conditioned by the autoactivated R protein snc1 (for suppressor of npr1-1, constitutive 1). mos7-1 single mutant plants exhibit defects in basal and R protein-mediated immunity and in systemic acquired resistance but do not display obvious pleiotropic defects in development, salt tolerance, or plant hormone responses. MOS7 is homologous to human and Drosophila melanogaster nucleoporin Nup88 and resides at the nuclear envelope. In animals, Nup88 attenuates nuclear export of activated NF-kappa B transcription factors, resulting in nuclear accumulation of NF-kappa B. Our analysis shows that nuclear accumulation of snc1 and the defense signaling components Enhanced Disease Susceptibility 1 and Nonexpresser of PR genes 1 is significantly reduced in mos7-1 plants, while nuclear retention of other tested proteins is unaffected. The data suggest that specifically modulating the nuclear concentrations of certain defense proteins regulates defense outputs.
引用
收藏
页码:2503 / 2516
页数:14
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