A method for isolating and analyzing human mRNA from newborn stool

被引：9

作者：

Bennett, William E., Jr. ^{[1
]}

Gonzalez-Rivera, Rosbel ^{[1
]}

Shaikh, Nurmohammad ^{[1
]}

Magrini, Vincent ^{[3
]}

Boykin, Michelle ^{[2
]}

Warner, Barbara B. ^{[2
]}

Hamvas, Aaron ^{[2
]}

Tarra, Phillip I.

机构：

[1] Washington Univ, Sch Med, Dept Pediat, Div Pediat Gastroenterol, St Louis, MO 63130 USA

[2] Washington Univ, Sch Med, Dept Pediat, Div Newborn Med, St Louis, MO 63130 USA

[3] Washington Univ, Genome Ctr, St Louis, MO 63130 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 349卷 / 1-2期

关键词：

Stool; Transcriptome; RNA; Nucleic acid purification; Newborn; POLYMERASE-CHAIN-REACTION; ESCHERICHIA-COLI; EXPRESSION; SAMPLES; CANCER; SALIVA; BLOOD;

D O I：

10.1016/j.jim.2009.07.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Efforts to characterize the human transcriptome have largely been limited to blood, urine, and tissue analyses (i.e., normally sterile materials). We report here an extraction protocol using commercially available reagents to obtain high-yield, reverse-transcribable RNA from human stool. Quantitative reverse transcriptase polymerase chain reactions demonstrated minimal intra-specimen but considerable intra-subject variability over time of transcripts for interleukin-6 (IL-6). IL-8, epidermal growth factor (EGF), calprotectin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This technique now expands opportunities to use the human fecal transcriptome to characterize gastrointestinal pathophysiology. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：56 / 60

页数：5

共 15 条

[1] Abu Al-Soud W, 1998, APPL ENVIRON MICROB, V64, P3748
[2] Ahmed Farid E., 2007, Cancer Genomics & Proteomics, V4, P1
[3] Improved methods for extracting RNA from exfoliated human colonocytes in stool and RT-PCR analysis
Ahmed, FE
James, SI
Lysle, DT
Dobbs, LJ
Johnke, RM
Flake, G
Stockton, P
Sinar, DR
Naziri, W
Evans, MJ
Kovacs, CJ
Allison, RR
[J]. DIGESTIVE DISEASES AND SCIENCES, 2004, 49 (11-12) : 1889 - 1898
[4] Purification of total RNA from human stool samples
Alexander, RJ
Raicht, RF
[J]. DIGESTIVE DISEASES AND SCIENCES, 1998, 43 (12) : 2652 - 2658
[5] BOKETE TN, 1993, GASTROENTEROLOGY, V105, P1724, DOI 10.1016/0016-5085(93)91069-T
[6] Integrating Global Gene Expression Analysis and Genetics
Farber, Charles R.
Lusis, Aldons J.
[J]. GENETIC DISSECTION OF COMPLEX TRAITS, 2ND EDITION, 2008, 60 : 571 - 601
[7] An Efficient RNA Extraction Method for Estimating Gut Microbial Diversity by Polymerase Chain Reaction
Kang, Seungha
Denman, Stuart E.
Morrison, Mark
Yu, Zhongtang
McSweeney, Chris S.
[J]. CURRENT MICROBIOLOGY, 2009, 58 (05) : 464 - 471
[8] Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
Koonjul, PK
Brandt, WF
Farrant, JM
Lindsey, GG
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (03) : 915 - 916
[9] Validity of messenger RNA expression analyses of human saliva
Kumar, Shalini V.
Hurteau, Gregory J.
Spivack, Simon D.
[J]. CLINICAL CANCER RESEARCH, 2006, 12 (17) : 5033 - 5039
[10] The peripheral-blood transcriptome: new insights into disease and risk assessment
Mohr, Steve
Liew, Choong-Chin
[J]. TRENDS IN MOLECULAR MEDICINE, 2007, 13 (10) : 422 - 432

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