Anti-DNA antibodies - overview of assays and clinical correlations

被引:25
作者
Rahman, A
Hiepe, F
机构
[1] UCL, Dept Med, Ctr Rheumatol, London WIT 4NJ, England
[2] Humboldt Univ, Charite, Med Klin 3, D-10117 Berlin, Germany
关键词
anti-DNA; nucleosomel; disease activity; apoptosis;
D O I
10.1191/0961203302lu313rp
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Many authors have studied the links between levels of anti-dsDNA antibodies and disease activity in patients with SLE. Interpretation of these studies must take into account the facts that there are a range of possible assays for anti-dsDNA and a number of indices available for assessing disease activity. A recent study compared levels of various autoantibodies with organ specific disease activity assessed during the British Isles Lupus Assessment Group (BILAG) index. Amti-dsDNA and anti-heparan sulphate levels were more likely to be raised in patients with renal than non-renal disease. Some anti-DNA antibodies are actually anti-nucleosome antibodies, which lose DNA reactivity when purified under dissociating conditions. Patients with SLE have significantly increased levels of nucleosomes in their sera compared with healthy controls. In patients with SLE, reduced clearance of nucleosomes released from apoptotic cells may induce the formation of anti-nucleosome antibodies.
引用
收藏
页码:770 / 773
页数:4
相关论文
共 14 条
  • [1] CLINICAL-EVALUATION OF A MODIFIED ELISA, USING PHOTOBIOTINYLATED DNA, FOR THE DETECTION OF ANTI-DNA ANTIBODIES
    HYLKEMA, MN
    HUYGEN, H
    KRAMERS, C
    VANDERWAL, TJ
    DEJONG, J
    VANBRUGGEN, MCJ
    SWAAK, AJG
    BERDEN, JHM
    SMEENK, RJT
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 170 (01) : 93 - 102
  • [2] Histone-containing immune complexes are to a large extent responsible for anti-dsDNA reactivity in the Farr assay of active SLE patients
    Hylkema, MN
    van Bruggen, MCJ
    ten Hove, T
    de Jong, J
    Swaak, AJG
    Berden, JHM
    Smeenk, RJT
    [J]. JOURNAL OF AUTOIMMUNITY, 2000, 14 (02) : 159 - 168
  • [3] Assessing patients with lupus: towards a drug responder index
    Isenberg, D
    Ramsey-Goldman, R
    [J]. RHEUMATOLOGY, 1999, 38 (11) : 1045 - 1049
  • [4] MEASUREMENT OF ANTI-DNA ANTIBODIES - A REAPPRAISAL USING 5 DIFFERENT METHODS
    ISENBERG, DA
    DUDENEY, C
    WILLIAMS, W
    ADDISON, I
    CHARLES, S
    CLARKE, J
    TODDPOKROPEK, A
    [J]. ANNALS OF THE RHEUMATIC DISEASES, 1987, 46 (06) : 448 - 456
  • [5] Isenberg DA, 1997, BRIT J RHEUMATOL, V36, P229
  • [6] From BILAG to BLIPS - disease activity assessment in lupus past, present and future
    Isenberg, DA
    Gordon, C
    [J]. LUPUS, 2000, 9 (09) : 651 - 654
  • [7] The role of antibodies to DNA in systemic lupus erythematosus - A review and introduction to an International Workshop on DNA Antibodies held in London, May 1996
    Isenberg, DA
    Ravirajan, CT
    Rahman, A
    Kalsi, J
    [J]. LUPUS, 1997, 6 (03) : 290 - 304
  • [8] LACK OF SPECIFICITY FOR ANTIBODIES TO DOUBLE-STRANDED DNA FOUND IN 4 COMMERCIAL KITS
    KADLUBOWSKI, M
    JACKSON, M
    YAP, PL
    NEILL, G
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1991, 44 (03) : 246 - 250
  • [9] STUDIES ON AUTOANTIBODIES TO POLY (ADENOSINE DIPHOSPHATE-RIBOSE) IN SLE AND OTHER AUTOIMMUNE-DISEASES
    MORROW, WJW
    ISENBERG, DA
    PARRY, HF
    SHEN, L
    OKOLIE, EE
    FARZANEH, F
    SHALL, S
    SNAITH, ML
    [J]. ANNALS OF THE RHEUMATIC DISEASES, 1982, 41 (04) : 396 - 402
  • [10] An analysis of clinical disease activity and nephritis-associated serum autoantibody profiles in patients with systemic lupus erythematosus: a cross-sectional study
    Ravirajan, CT
    Rowse, L
    MacGowan, JR
    Isenberg, DA
    [J]. RHEUMATOLOGY, 2001, 40 (12) : 1405 - 1412