Overexpression of nreB, a new GATA factor-encoding gene of Penicillium chrysogenum, leads to repression of the nitrate assimilatory gene cluster

被引:41
作者
Haas, H
Angermayr, K
Zadra, I
Stoffler, G
机构
[1] Dept. of Microbiol. (Medical School), University of Innsbruck, A-6020 Innsbruck
关键词
D O I
10.1074/jbc.272.36.22576
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate the mechanism of nitrogen metabolite repression in the biotechnologically important fungus Penicillium chrysogenum a polymerase chain reaction approach was employed to identify transcription factors involved in this regulatory circuit, leading to the isolation of a new gene (nreB) encoding a 298 amino acid protein. Despite a low overall amino acid sequence identity of approximately 30%, it shares several features with Dal80p/Uga43p and Gzf3p/Nil2p, both repressors in nitrogen metabolism in Saccharomyces cerevisiae. All three proteins contain an N-terminal GATA-type zinc finger motif, displaying 86% amino acid sequence identity, and a putative leucine zipper motif in the C terminus. Northern blot analysis revealed the presence of two nreB transcripts, 1.8 and 1.5 kilobases in length, that differ in polyadenylation sites. The steady state level of both transcripts is subject to nitrogen metabolite repression. The putative DNA binding domain of NREB, expressed as a fusion protein in Escherichia coli, binds in vitro to GATA sites of its own 5'-upstream region as well as in the promoter of the nitrate assimilation gene cluster. Consistent with a role in the regulation of nitrogen metabolism, overexpression of nreB leads to repression of nitrate assimilatory genes. Hence, the simple view of nitrogen regulation by four GATA factors in yeast, but only one key regulator in filamentous ascomycetes seems no longer valid.
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页码:22576 / 22582
页数:7
相关论文
共 49 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]  
[Anonymous], [No title captured]
[3]   A NEW-GENERATION OF INFORMATION-RETRIEVAL TOOLS FOR BIOLOGISTS - THE EXAMPLE OF THE EXPASY WWW SERVER [J].
APPEL, RD ;
BAIROCH, A ;
HOCHSTRASSER, DF .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (06) :258-260
[4]   Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae [J].
Blinder, D ;
Coschigano, PW ;
Magasanik, B .
JOURNAL OF BACTERIOLOGY, 1996, 178 (15) :4734-4736
[5]  
Caddick Mark X., 1997, Genes and Function, V1, P37
[6]   HIGH-FREQUENCY TRANSFORMATION OF PENICILLIUM-CHRYSOGENUM [J].
CANTORAL, JM ;
DIEZ, B ;
BARREDO, JL ;
ALVAREZ, E ;
MARTIN, JF .
BIO-TECHNOLOGY, 1987, 5 (05) :494-497
[7]  
CHAE KS, 1995, MOL CELLS, V5, P146
[8]  
Coffman JA, 1996, MOL CELL BIOL, V16, P847
[9]  
COOPER TG, 1996, MYCOTA, V3, P139
[10]   THE UGA43 NEGATIVE REGULATORY GENE OF SACCHAROMYCES-CEREVISIAE CONTAINS BOTH A GATA-1 TYPE ZINC FINGER AND A PUTATIVE LEUCINE ZIPPER [J].
COORNAERT, D ;
VISSERS, S ;
ANDRE, B ;
GRENSON, M .
CURRENT GENETICS, 1992, 21 (4-5) :301-307