Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramide signal amplification

被引：18

作者：

Strappe, PM ^{[1
]}

Wang, TH ^{[1
]}

McKenzie, CA ^{[1
]}

Lowrie, S ^{[1
]}

Simmonds, P ^{[1
]}

Bell, JE ^{[1
]}

机构：

[1] UNIV EDINBURGH, DEPT MED MICROBIOL, EDINBURGH EH8 9AG, MIDLOTHIAN, SCOTLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 67卷 / 01期

基金：

英国医学研究理事会;

关键词：

human immunodeficiency virus type 1; p24; antigen; immunohistochemistry; tyramide signal amplification; brain tissue;

D O I：

10.1016/S0166-0934(97)00083-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human immunodeficiency virus type 1 (HIV-1) infection of the brain has been demonstrated in formalin fixed, paraffin embedded post-mortem brain tissue (PM) by chromogenic immunohistochemistry for the HIV p24 antigen. The sensitivity of antigen detection is increased significantly by tyramide signal amplification (TSA) compared to the conventional peroxidase labelled Avidin-Biotin complex (ABC) technique. The TSA method also permitted the use of a lower concentration of primary antibody than is conventionally used. Sensitivity was enhanced further by microwave irradiation of the paraffin embedded tissues in citrate buffer. HIV-1 p24 antigen was also detected in PM brain tissue by TSA enhanced immunofluorescence and demonstrated increased sensitivity compared to the conventional immunofluorescence technique with a greatly reduced autofluorescence background. (C) 1997 Elsevier Science B.V.

引用

页码：103 / 112

页数：10

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