Simultaneous determination of α-tocopherol and β-carotene in olive oil by reversed-phase high-performance liquid chromatography

被引：83

作者：

Gimeno, E ^{[1
]}

Calero, E ^{[1
]}

Castellote, A ^{[1
]}

Lamuela-Raventós, RM ^{[1
]}

de la Torre, MC ^{[1
]}

López-Sabater, MC ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Ctr Referencia Tecnol Aliments, CeRTA,Dpt Nutr Bromatol, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 881卷 / 1-2期

关键词：

olive oil; food analysis; vitamins; tocopherols; carotenes; antioxidants;

D O I：

10.1016/S0021-9673(00)00272-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic method was developed for the determination, in one run, of alpha-tocopherol and beta-carotene in virgin olive oil. The method involved a rapid saponification and a later extraction with a mixture of hexane-ethyl acetate. The chromatographic system consists of an ODS-2 column with a mobile phase of methanol-water-butanol and a diode-array detector. Linearity, precision, recovery and sensitivity were satisfactory. The main advantage of the proposed method is the speed and simultaneous determination of both compounds at the same time. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：255 / 259

页数：5

共 35 条

[1] Antioxidant activity of tocopherols and phenolic compounds of virgin olive oil [J].