Au@Fe3O4 nanoparticles;
Hemoglobin;
Hydrogen peroxide;
Direct electrochemistry;
DIRECT ELECTRON-TRANSFER;
HYDROGEN-PEROXIDE;
ELECTROCATALYSIS;
BIOSENSOR;
FILM;
IMMOBILIZATION;
CHITOSAN;
D O I:
10.1007/s00604-009-0192-9
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Nanoparticles (NPs) consisting of an Fe3O4 core and a thin gold shell (referred to as Au@Fe3O4 NPs) were self-assembled on the surface of a glassy carbon electrode modified with ethylenediamine. Following adsorption of hemoglobin, its interaction with the NPs was studied by UV-Vis spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Stable and well-defined redox peaks were observed at about -350 mV and -130 mV in pH 6.0 buffer. The modified electrode was used as a mediator-free sensor for hydrogen peroxide (H2O2), with a linear range from 3.4 A mu M to 4.0 mM of H2O2, and with a 0.67 A mu M detection limit (at an S/N of 3). The apparent Michaelis-Menten constant is 2.3 mM.
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Jia, Nengqin
Lian, Qiong
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机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Lian, Qiong
Wang, Zhiyong
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机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Wang, Zhiyong
Shen, Hebai
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h-index: 0
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Jia, Nengqin
Lian, Qiong
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Lian, Qiong
Wang, Zhiyong
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
Wang, Zhiyong
Shen, Hebai
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China