Direct electrochemical behavior of hemoglobin at surface of Au@Fe3O4 magnetic nanoparticles

被引：43

作者：

Yu, Chun-Mei ^{[1
,2
]}

Guo, Jian-Wei ^{[1
]}

Gu, Hai-Ying ^{[1
]}

机构：

[1] Nantong Univ, Sch Publ Hlth, Nantong 226007, Peoples R China

[2] Soochow Univ, Coll Chem & Chem Engn & Mat Sci, Suzhou 215123, Peoples R China

来源：

MICROCHIMICA ACTA | 2009年 / 166卷 / 3-4期

关键词：

Au@Fe3O4 nanoparticles; Hemoglobin; Hydrogen peroxide; Direct electrochemistry; DIRECT ELECTRON-TRANSFER; HYDROGEN-PEROXIDE; ELECTROCATALYSIS; BIOSENSOR; FILM; IMMOBILIZATION; CHITOSAN;

D O I：

10.1007/s00604-009-0192-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nanoparticles (NPs) consisting of an Fe3O4 core and a thin gold shell (referred to as Au@Fe3O4 NPs) were self-assembled on the surface of a glassy carbon electrode modified with ethylenediamine. Following adsorption of hemoglobin, its interaction with the NPs was studied by UV-Vis spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Stable and well-defined redox peaks were observed at about -350 mV and -130 mV in pH 6.0 buffer. The modified electrode was used as a mediator-free sensor for hydrogen peroxide (H2O2), with a linear range from 3.4 A mu M to 4.0 mM of H2O2, and with a 0.67 A mu M detection limit (at an S/N of 3). The apparent Michaelis-Menten constant is 2.3 mM.

引用

页码：215 / 220

页数：6

共 29 条

[1] Direct electron transfer and characterization of hemoglobin immobilized on a Au colloid-cysteamine-modified gold electrode
Gu, HY
Yu, AM
Chen, HY
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2001, 516 (1-2): : 119 - 126
[2] The immobilization of hepatocytes on 24 nm-sized gold colloid for enhanced hepatocytes proliferation
Gu, HY
Chen, Z
Sa, RX
Yuan, SS
Chen, HY
Ding, YT
Yu, AM
[J]. BIOMATERIALS, 2004, 25 (17) : 3445 - 3451
[3] Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor
Guo, Chunxian
Hu, Fengping
Li, Chang Ming
Shen, Pei Kang
[J]. BIOSENSORS & BIOELECTRONICS, 2008, 24 (04) : 819 - 824
[4] Assembly of layer-by-layer films of electroactive hemoglobin and surfactant didodecyldimethylammonium bromide
Hu, Yang
Sun, Hong
Hu, Naifei
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2007, 314 (01) : 131 - 140
[5] Chitosan mediated assembly of gold nanoparticles multilayer
Huang, HZ
Yang, XR
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2003, 226 (1-3) : 77 - 86
[6] A hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin incorporated in PEO-PPO-PEO triblock copolymer film
Jia, Nengqin
Lian, Qiong
Wang, Zhiyong
Shen, Hebai
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2009, 137 (01) : 230 - 234
[7] Development of a peroxide biosensor made of a thiolated-viologen and hemoglobin-modified gold electrode
Kafi, A. K. M.
Lee, Dong-Yun
Park, Sang-Hyun
Kwon, Young-Soo
[J]. MICROCHEMICAL JOURNAL, 2007, 85 (02) : 308 - 313
[8] ROTATING-RING-DISK ENZYME ELECTRODE FOR BIOCATALYSIS KINETIC-STUDIES AND CHARACTERIZATION OF THE IMMOBILIZED ENZYME LAYER
KAMIN, RA
WILSON, GS
[J]. ANALYTICAL CHEMISTRY, 1980, 52 (08) : 1198 - 1205
[9] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS
LAVIRON, E
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) : 19 - 28
[10] Amperometric detection of nitrite using a nanometer-sized gold colloid modified pretreated glassy carbon electrode
Liu, Yang
Gu, Hai-Ying
[J]. MICROCHIMICA ACTA, 2008, 162 (1-2) : 101 - 106

← 1 2 3 →