High-throughput assay of (R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase

被引：20

作者：

Breuer, M

Pohl, M

Hauer, B

Lingen, B

机构：

[1] BASF AG, D-67056 Ludwigshafen, Germany

[2] MBP Cologne GmbH, D-51063 Cologne, Germany

[3] Univ Dusseldorf, Inst Enzymtechnol, Forschungszentrum, D-52426 Julich, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 374卷 / 06期

关键词：

high-throughput assay; (R)-phenylacetylcarbinol; pyruvate decarboxylase; tetrazolium red;

D O I：

10.1007/s00216-002-1579-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel assay has been developed for the detection of (R)-phenylacetylcarbinol, (R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.

引用

页码：1069 / 1073

页数：5

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