The adjuvant monophosphoryl lipid A increases the function of antigen-presenting cells

被引:136
作者
De Becker, G
Moulin, V
Pajak, B
Bruck, C
Francotte, M
Thiriart, C
Urbain, J
Moser, M
机构
[1] Free Univ Brussels, Dept Biol Mol, B-6041 Gosselies, Belgium
[2] Smith Kline Beecham Biol, B-1330 Rixensart, Belgium
关键词
adjuvant; in vivo animal models; primary response; T(h)1/T(h)2;
D O I
10.1093/intimm/12.6.807
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The induction of immune responses in vivo is typically performed with antigens administered in external adjuvants, like alum, complete Freund's adjuvant, LPS and, more recently, monophosphoryl lipid A (MPL), However, the role of the adjuvant is still poorly defined. The aim of this study was to test whether the MPL affects the function of antigen-presenting cells (APC) in vitro and In vivo. Antigen-pulsed APC [including macrophages, B cells and dendritic cells (DC)] were incubated or not with MPL, and their ability to sensitize naive T cells was tested in vitro and In vivo. The data show that MPL enhances the ability of macrophages and B cells to sensitize naive T cells, and confers to them the capacity to induce the development of T(h)1 and T(h)2. Administration of MPL i.v. In mice results in the redistribution of fully mature DC in the T cell area of the spleen, These observations suggest that MPL may induce an antigen-specific primary immune response by provoking the migration and maturation of DC that are the physiological adjuvant of the immune system.
引用
收藏
页码:807 / 815
页数:9
相关论文
共 36 条
  • [1] Dendritic cells and the control of immunity
    Banchereau, J
    Steinman, RM
    [J]. NATURE, 1998, 392 (6673) : 245 - 252
  • [2] THE ROLE OF HELPER T-CELL PRODUCTS IN MOUSE B-CELL DIFFERENTIATION AND ISOTYPE REGULATION
    COFFMAN, RL
    SEYMOUR, BWP
    LEBMAN, DA
    HIRAKI, DD
    CHRISTIANSEN, JA
    SHRADER, B
    CHERWINSKI, HM
    SAVELKOUL, HFJ
    FINKELMAN, FD
    BOND, MW
    MOSMANN, TR
    [J]. IMMUNOLOGICAL REVIEWS, 1988, 102 : 5 - 28
  • [3] CROFTON J, 1992, AMORPHOUS CRYSTALLIN, V4, P176
  • [4] DENDRITIC CELLS ARE THE PRINCIPAL CELLS IN MOUSE SPLEEN BEARING IMMUNOGENIC FRAGMENTS OF FOREIGN PROTEINS
    CROWLEY, M
    INABA, K
    STEINMAN, RM
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) : 383 - 386
  • [5] De Becker G, 1998, EUR J IMMUNOL, V28, P3161, DOI 10.1002/(SICI)1521-4141(199810)28:10<3161::AID-IMMU3161>3.3.CO
  • [6] 2-H
  • [7] DeBecker G, 1997, ADV EXP MED BIOL, V417, P369
  • [8] IMMUNOGLOBULIN ISOTYPE REGULATION BY ANTIGEN-PRESENTING CELLS IN-VIVO
    DEBECKER, G
    SORNASSE, T
    NABAVI, N
    BAZIN, H
    TIELEMANS, F
    URBAIN, J
    LEO, O
    MOSER, M
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (07) : 1523 - 1528
  • [9] Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo
    DeSmedt, T
    Pajak, B
    Muraille, E
    Lespagnard, L
    Heinen, E
    DeBaetselier, P
    Urbain, J
    Leo, O
    Moser, M
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (04) : 1413 - 1424
  • [10] Faquim-Mauro EL, 1999, J IMMUNOL, V163, P3572