Pollutant sensing layer based on cytochrome P450

被引：19

作者：

Paternolli, C ^{[1
]}

Ghisellini, P ^{[1
]}

Nicolini, C ^{[1
]}

机构：

[1] Univ Genoa, Dept Biophys M&O Sci & Technol, I-16132 Genoa, Italy

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2002年 / 22卷 / 02期

关键词：

Langnmir-Blodgett films; UV-VIS spectroscopy; nanogravimetry; styrene;

D O I：

10.1016/S0928-4931(02)00164-9

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this work, cytochrome P4502134 fusion protein was utilized to realize sensing layers for detection of styrene in atmosphere. Glutathione S-transferase (GST) fusion protein was utilized to realize thin films by Langmuir-Blodgett (LB) horizontal transfer technique. The interaction between the styrene and the sensing layer was monitored by spectrophotometric and by gravimetric measurements. A shift of the Soret peak of cytochrome P450 in the presence of the substrate was found when absorbance measurements were performed. Index of spin state equilibrium variation of P4502134 was calculated in order to verify the interaction between styrene and cytochrome. A saturation trend in mass density was found when quartzes nanobalance was utilized. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：155 / 159

页数：5

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