DRB3 alleles with variations in the annealing sites of commonly used amplification primers

被引:7
作者
Coquillard, GJ
Tang, TF
Steiner, N
Perlee, L
Ng, J
Hartzman, R
Hurley, CK
机构
[1] Georgetown Univ, Med Ctr, Dept Pediat, Washington, DC 20007 USA
[2] Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20007 USA
[3] Lifecodes Corp, Stamford, CT USA
[4] USN, Med Res Ctr, Bethesda, MD 20084 USA
来源
TISSUE ANTIGENS | 2000年 / 55卷 / 06期
关键词
class II; direct DNA sequencing; DRB3; alleles; human leukocyte antigen (HLA);
D O I
10.1034/j.1399-0039.2000.550606.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
New HLA alleles are often identified initially from observing uncommon patterns found in low-resolution typing performed via polymerase chain reaction using sequence-specific oligonucleotide probes (PCRSSOP). Recently, the HLA-DR oligotyping analysis of two Caucasian, one Caucasian/American Indian and two African American individuals resulted in the identification of three novel DRB3 alleles Using DRB-specific primer sets commonly employed in amplification-based typing, all four individuals were originally characterized as DRB3 negative. Direct sequencing identified DRB3*0104 (variation at codon 8, TCG instead of TTG), and DRB3*0101202 (variation at intron (-13), G instead of C). One individual appeared to carry a DR52-associated DRB1 allele without an associated DRB3 allele. Lack of conservation at the junction of intron 1 and exon 2 of the DRB3 gene suggests that commonly used DRB-specific primer sets may need to be modified.
引用
收藏
页码:558 / 563
页数:6
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