Production of interspecific F-1 hybrids, BC1, BC2 and BC3 populations between Lycopersicon esculentum and two accessions of Lycopersicon peruvianum carrying new root-knot nematode resistance genes

被引：19

作者：

Doganlar, S ^{[1
]}

Frary, A ^{[1
]}

Tanksley, SD ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT PLANT BREEDING & BIOMETRY, ITHACA, NY 14853 USA

来源：

EUPHYTICA | 1997年 / 95卷 / 02期

关键词：

crossability barriers; ovule culture; interspecific crosses; Lycopersicon esculentum; Lycopersicon peruvianum;

D O I：

10.1023/A:1002949700854

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Two accessions of Lycopersicon peruviantum Mill. (PI270435, PI126443) carrying Mi-2 and Mi-3, respectively, new root-knot nematode resistance genes, were selected as the male parents for crosses with L. Esculentum Mill. in order to produce interspecific hybrids. Crossability barriers between these two distantly related species were circumvented by ovule culture. A total of ten interspecific F-1 hybrid plants were produced. The hybrid nature of the putative F-1 plants was confirmed by a comparison of several morphological characteristics and a PCR-based assay. Eight of ten hybrid plants were backcrossed with L. esculentum to generate a total of 98 BC1 progeny. Two lines were advanced to the BC2 and BC3 levels. Based on these results, ovule culture was found to be an effective method for the production of novel interspecific F-1 and BC1 plants.

引用

页码：203 / 207

页数：5

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