Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses

被引：56

作者：

Alberini, Isabella ^{[1
]}

Del Tordello, Elena ^{[1
]}

Fasolo, Alba ^{[1
]}

Temperton, Nigel J. ^{[2
]}

Galli, Grazia ^{[1
]}

Gentile, Chiara ^{[3
]}

Montomoli, Emanuele ^{[3
]}

Hilbert, Anne K. ^{[4
]}

Banzhoff, Angelika ^{[4
]}

Del Giudice, Giuseppe ^{[1
]}

Donnelly, John J. ^{[1
]}

Rappuoli, Rino ^{[1
]}

Capecchi, Barbara ^{[1
]}

机构：

[1] Novartis Vaccines, Siena, Italy

[2] UCL, Ctr Med Mol Virol, Div Infect & Immun, London WC1E 6BT, England

[3] Univ Siena, Dept Physiopathol Expt Med & Publ Hlth, Lab Mol Epidemiol, I-53100 Siena, Italy

[4] Novartis Vaccines, Marburg, Germany

来源：

VACCINE | 2009年 / 27卷 / 43期

关键词：

Pseudoparticle neutralization assay; Avian influenza virus; H5N1; Pandemic vaccine; Cross-clade neutralization; Serological methods; SINGLE-RADIAL-HEMOLYSIS; MF59-ADJUVANTED INFLUENZA; A H5N1; RANDOMIZED-TRIAL; VACCINE; ANTIBODY; INFECTION; HEMAGGLUTININ; RESPONSES; SAFETY;

D O I：

10.1016/j.vaccine.2009.07.079

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticle neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity. (C) 2009 Elsevier Ltd. All rights reserved

引用

页码：5998 / 6003

页数：6

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