In vivo modulation of epidermal growth factor receptor phosphorylation in mice expressing different Gangliosides

被引：13

作者：

Daniotti, Jose L.

Crespo, Pilar M.

Yamashita, Tadashi

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, CONICET, Ctr Invest Quim Biol Cordoba,CIQUIBIC,Dept Quim B, RA-5000 Cordoba, Argentina

[2] NIDDK, Genet Dev & Dis Branch, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2006年 / 99卷 / 05期

关键词：

gangliosides; EGF receptor; ganglioside glycosyltransferase; skin; mice; glycolipid;

D O I：

10.1002/jcb.21034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied in this work the in vivo phosphorylation of the epidermal growth factor receptor (EGFr) in skin from knockout mice lacking different ganglioside glycosyltransferases. Results show an enhancement of EGFr phosphorylation, after EGF stimulation, in skin from Sial-T2 knockout and Sial-T2/GaINAc-T double knockout mice as compared with wild-type and Sial-Ti knockout mice. Qualitative analysis of ganglioside composition in mice skin suggest that the increase of EGFr phosphorylation observed in skin from Sial-T2 knockout and Sial-T2/Gal NA&T double knockout mice in response to EGF might not be primary attributed to the expression of GD3 or a-series gangliosides in mice skin. These studies provide, for the first time, an approach for studying the molecular mechanisms involved in the in vivo regulation of EGFr function by gangliosides. J. Cell. Biochem. 99: 1442-1451, 2006. (c) 2006 Wiley-Liss, Inc.

引用

页码：1442 / 1451

页数：10

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