Application of directly coupled high performance liquid chromatography-NMR-mass spectometry and 1H NMR spectroscopic studies to the investigation of 2,3-benzofuran metabolism in Sprague-Dawley rats

被引：13

作者：

Connelly, JC ^{[1
]}

Connor, SC ^{[1
]}

Monte, S ^{[1
]}

Bailey, NJC ^{[1
]}

Borgeaud, N ^{[1
]}

Holmes, E ^{[1
]}

Troke, J ^{[1
]}

Nicholson, JK ^{[1
]}

Gavaghan, CL ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Biol Sci, Dept Biol Chem, London, England

来源：

DRUG METABOLISM AND DISPOSITION | 2002年 / 30卷 / 12期

关键词：

D O I：

10.1124/dmd.30.12.1357

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

(T)he urinary excretion of metabolites of 2,3-benzofuran was studied in Sprague-Dawley rats (n = 5) given a single dose of 150 mg/kg i.p. Urine samples were collected at defined intervals up to 7 days postdose and analyzed using H-1 NMR and directly coupled high performance liquid chromatography ( HPLC)-NMR, HPLC-(mass spectrometry) MS and HPLC-MS-NMR methods. The principal metabolites were determined to be 2-hydroxyphenylacetic acid and 2-(2-hydroxyethyl) phenyl hydrogen sulfate, representing 24.3 +/- 6.0% and 19.6 +/- 6.4% of the dose, respectively. This indicates that metabolism of benzofuran to the polar species excreted in urine involves cleavage of the furan ring.

引用

页码：1357 / 1363

页数：7

共 22 条

[1] Characterization of metabolites in intact Streptomyces citricolor culture supernatants using high-resolution nuclear magnetic resonance and directly coupled high-pressure liquid chromatography-nuclear magnetic resonance spectroscopy [J].