Respiratory syncytial virus enhances respiratory allergy in mice despite the inhibitory effect of virus-induced interferon-γ

被引:12
作者
Barends, M
Boelen, A
de Rond, L
Dormans, J
Kwakkel, J
van Oosten, M
Neijens, HJ
Kimman, TG
机构
[1] Natl Inst Publ Hlth & Environm, Res Lab Infect Dis, NL-3720 BA Bilthoven, Netherlands
[2] Natl Inst Publ Hlth & Environm, Lab Pathol & Immunobiol, NL-3720 BA Bilthoven, Netherlands
[3] Erasmus Univ, Dept Paediat, Rotterdam, Netherlands
关键词
IFN-gamma; IL-12; RSV; Th2; response; eosinophils; mice;
D O I
10.1002/jmv.10252
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In mice, respiratory syncytial virus (RSV) infection during allergic provocation aggravates the allergic Th2 immune response, characterised by production of interleukin (IL)-4, IL-5, and IL-13, and eosinophilic inflammation. This enhancement of the Th2 response occurs simultaneously with a strong RSV-induced Th1 cytokine response (IL-12 and IFN-gamma). The present study investigated whether IFN-gamma and IL-12 are critically involved in this RSV-enhanced OVA allergy. Therefore, IFN-gammaR- and IL-12-deficient mice (both on a 129/Sv/Ev background) were sensitised and challenged with ovalbumin (OVA) and infected with RSV during the OVA challenge period. Neither gene deletion affected the development of ovalbumin-induced allergic inflammation in mice. However, when OVA-allergic IFN-gammaR deficient mice were infected with RSV, an increased pulmonary eosinophilic infiltrate and increased IL-4 and IL-13 mRNA expression in lung tissue were observed compared with identically treated wild-type mice. In contrast, deficiency of IL-12 did not aggravate the Th2 immune and inflammatory response in OVA/RSV-treated mice, compared with Wild-type. In conclusion, the virus-induced IFN-gamma response diminishes the Th2 inflammatory response during OVA allergy but fails to prevent totally the enhancement of the OVA allergy by RSV. In contrast, IL-12 is not involved in inhibiting nor increasing the RSV-enhanced allergy in 129/Sv/Ev mice. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:156 / 162
页数:7
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