Identification and transcriptional characterization of the gene encoding the stress-response σ factor σH in Streptomyces coelicolor A3(2)

被引：31

作者：

Kormanec, J ^{[1
]}

Sevcíková, B ^{[1
]}

Halgasová, N ^{[1
]}

Knirschová, R ^{[1
]}

Rezuchová, B ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Mol Biol, Bratislava 84251, Slovakia

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 189卷 / 01期

关键词：

recombinant DNA; differentiation; stress response; RNA polymerase; sigma factor; anti-sigma factor; Na/H antiporter; sequence comparison; transcriptional analysis; Streptomyces;

D O I：

10.1111/j.1574-6968.2000.tb09202.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned a new gene, sigH, encoding an alternative a factor in Streptomyces coelicolor A3(2). The deduced protein of 354 amino acids with an M-r of 39 486 showed greatest similarity to the sporulation sigma factor (sigma(F)) of S. coelicolor, general stress-response sigma(B) of Bacillus subtilis, and stationary-phase stress-response sigma(F) of Mycobacterium tuberculosis. Sequence analysis of the upstream region revealed an ORF encoding a protein (UshX) similar to several anti-a factors, and short ORF (UshY) containing zinc-finger DNA binding motif. Transcriptional analysis revealed that all three genes are located on the same polycistronic transcript in order ushY, ushX, and sigH. Expression of the operon was directed by four promoters differentially expressed in the course of differentiation. The first (PI) constitutive promoter was located upstream of ush Y. The other three promoters (P2, P3, and P4) were located upstream of ushX, and were differentially induced after various stress conditions. The magnitude of the induction was greatest after osmotic stress and heat shock. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：31 / 38

页数：8

共 20 条

[1]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[2] Identification of the gene encoding the alternative sigma factor σB from Listeria monocytogenes and its role in osmotolerance [J].