A method for high-throughput gene expression signature analysis

被引：157

作者：

Peck, David

D Crawford, Emily

Ross, Kenneth N.

Stegmaier, Kimberly

Golub, Todd R.

Lamb, Justin ^{[1
]}

机构：

[1] MIT, Broad Inst, Cambridge, MA 02142 USA

[2] Howard Hughes Med Inst, Dana Farber Canc Inst, Boston, MA 02115 USA

来源：

GENOME BIOLOGY | 2006年 / 7卷 / 07期

关键词：

D O I：

10.1186/gb-2006-7-7-r61

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

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