Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium

被引：9

作者：

Barbieri, E

Riccioni, G

Pisano, A

Sisti, D

Zeppa, S

Agostini, D

Stocchi, V

机构：

[1] Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, Italy

[2] Univ Urbino, Giorgio Fornaini Inst Biochem, I-61029 Urbino, Italy

[3] Univ Urbino, Inst Bot, I-61029 Urbino, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 12期

关键词：

D O I：

10.1128/AEM.68.12.6421-6424.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.

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页码：6421 / 6424

页数：4

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