Interleukin-10 expression after intramuscular DNA electrotransfer: kinetic studies

被引：17

作者：

Deleuze, V ^{[1
]}

Scheman, D ^{[1
]}

Bureau, MF ^{[1
]}

机构：

[1] CNRS, ENSCP, UMR 7001, Gencell SA,Ctr Rech Vitry Alforville, F-94403 Vitry Sur Seine, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 299卷 / 01期

关键词：

interleukin-10; electrotransfer; kinetics study; skeletal muscle; electroporation gene therapy;

D O I：

10.1016/S0006-291X(02)02580-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfected muscle can be used as a secreting tissue for therapeutic proteins. Skeletal muscle transfection is increased by suitable electric pulse application (electrotransfer). We and others had shown that electrotransfer of interleukin-10 encoding plasmid is an effective strategy in animal models of chronic diseases such as myocarditis, atherosclerosis, or rheumatoid arthritis. In the present work, we have studied murine interleukin-10 production and secretion after i.m. electrotransfer. In immunocompetent mice, serum and muscle mIL-10 levels were enhanced by electrotransfer. Serum mIL-10 concentration reached rapidly a peak level 2 days after electrotransfer. It then decreased to background at day 14. Muscle mIL-10 mRNA and protein remained more stable, being detectable up to 84 days after electrotransfer. A boost reinjection led to similar high level of circulating mIL-10. The-fast decrease of serum mIL-10 was not observed in SCID mice. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：29 / 34

页数：6

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