Functional consequence of plasmid DNA modified site-specifically with 7-deaza-deoxyadenosine at a single, programmable site

被引：7

作者：

Ang, Wee Han ^{[1
]}

Lippard, Stephen J. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 39期

关键词：

RECOGNITION; PCR; 7-DEAZA-2'-DEOXYGUANOSINE; SEQUENCE;

D O I：

10.1039/b914843d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Replacement of a single dA nucleotide positioned at a programmed site in a DNA plasmid with its 7-deaza-analog is described together with its complete resistance to restriction enzymatic cleavage.

引用

页码：5820 / 5822

页数：3

共 14 条

[1] BstAPI, an ApaBI isoschizomer, cleaves DNA at 5'-GCANNNN down arrow NTGC-3' [J].

Abdurashitov, MA ;

Belichenko, OA ;

Shevchenko, AV ;

Dedkov, VS ;

Degtyarev, SK .

NUCLEIC ACIDS RESEARCH, 1997, 25 (12) :2301-2302

[2] Preparation of Mammalian Expression Vectors Incorporating Site-Specifically Platinated-DNA Lesions [J].

Ang, Wee Han ;

Brown, William Wesley ;

Lippard, Stephen J. .

BIOCONJUGATE CHEMISTRY, 2009, 20 (05) :1058-1063

[3] HMG-Domain protein recognition of cisplatin 1,2-intrastrand d(GpG) cross-links in purine-rich sequence contexts [J].

Cohen, SM ;

Mikata, Y ;

He, Q ;

Lippard, SJ .

BIOCHEMISTRY, 2000, 39 (38) :11771-11776

[4] INHIBITION OF RESTRICTION ENZYME CLEAVAGE OF DNA MODIFIED WITH 7-DEAZA-DGTP [J].

GRIME, SK ;

MARTIN, RL ;

HOLAWAY, BL .

NUCLEIC ACIDS RESEARCH, 1991, 19 (10) :2791-2791

[5] Photoaffinity Isolation and Identification of Proteins in Cancer Cell Extracts that Bind to Platinum-Modified DNA [J].

Guggenheim, Evan R. ;

Xu, Dong ;

Zhang, Christiana X. ;

Chang, Pamela V. ;

Lippard, Stephen J. .

CHEMBIOCHEM, 2009, 10 (01) :141-157

[6] PCR-mediated deletion of plasmid DNA [J].

Hansson, Mattlas D. ;

Rzeznicka, Kamila ;

Rosenback, Matilda ;

Hansson, Mats ;

Sirijovski, Nick .

ANALYTICAL BIOCHEMISTRY, 2008, 375 (02) :373-375

[7] 7-deaza-2′-deoxyguanosine allows PCR and sequencing reactions from CpG islands [J].

Jung, A ;

Ruckert, S ;

Frank, P ;

Brabletz, T ;

Kirchner, T .

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, 2002, 55 (01) :55-57

[8] Regioselective syntheses of 7-nitro-7-deazapurine nucleosides and nucleotides for efficient PCR incorporation [J].

Kawate, T ;

Allerson, CR ;

Wolfe, JL .

ORGANIC LETTERS, 2005, 7 (18) :3865-3868

[9] cis-diammine(pyridine)chloroplatinum(II), a monofunctional platinum(II) antitumor agent:: Uptake, structure, function, and prospects [J].

Lovejoy, Katherine S. ;

Todd, Ryan C. ;

Zhang, Shuzhong ;

McCormick, Michael S. ;

D'Aquino, J. Alejandro ;

Reardon, Joyce T. ;

Sancar, Aziz ;

Giacomini, Kathleen M. ;

Lippard, Stephen J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (26) :8902-8907

[10] Crystal structure of restriction endonuclease Bg/I bound to its interrupted DNA recognition sequence [J].

Newman, M ;

Lunnen, K ;

Wilson, G ;

Greci, J ;

Schildkraut, I ;

Phillips, SEV .

EMBO JOURNAL, 1998, 17 (18) :5466-5476

← 1 2 →