Evaluation of 2-SP transport medium for detection of Chlamydia trachomatis and Neisseria gonorrhoeae by two automated amplification systems and culture for chlamydia

被引:10
作者
Dubuis, O
GorgievskiHrisoho, M
Germann, D
Matter, L
机构
[1] Institute for Medical Microbiology, University of Berne, 3010 Berne
关键词
2-sucrose-phosphate medium; polymerase chain reaction; ligase chain reaction;
D O I
10.1136/jcp.50.11.947
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims-To assess the performance of 2-sucrose-phosphate based transport medium (2-SP) for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by an automated commercial polymerase chain reaction (PCR) and ligase chain reaction (LCR) compared to centrifugation culture on McCoy cells for C trachomatis. Second, to compare both amplification systems for initial diagnostic testing of a low prevalence population for sexually transmitted diseases. Methods-Four hundred and eighty one consecutive urogenital and conjunctival specimens were examined. All tests were performed on the same specimen collected with a dacron swab and transported in 2-SP medium. Samples that were positive by culture or by both PCR and LCR were considered to be true positives. Results-The prevalences of C trachomatis and of N gonorrhoeae were 2.7% and 0.4%, respectively. PCR had a resolved sensitivity and specificity of 100% and 99.8%, respectively, for C trachomatis, and 100% and 98.9%, respectively, for N gonorrhoeae. LCR was 100% sensitive and specific for both pathogens. The resolved sensitivity of the shell vial assay was 85%. No culture positive sample would have been missed by PCR or LCR. The inhibition rate for PCR was 4.8%. Conclusions-2-SP medium proved to be suitable for both PCR and LCR. It is not limited to any one test manufacturer and allows the performance of amplification techniques and viral and chlamydia culture from the same specimen. The LCR was more reliable than PCR on initial testing. However, hands on time is longer, and no amplification control is available for LCR.
引用
收藏
页码:947 / 950
页数:4
相关论文
共 29 条
  • [1] CLINICAL-EVALUATION OF A NEW POLYMERASE CHAIN-REACTION ASSAY FOR DETECTION OF CHLAMYDIA-TRACHOMATIS IN ENDOCERVICAL SPECIMENS
    BASS, CA
    JUNGKIND, DL
    SILVERMAN, NS
    BONDI, JM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) : 2648 - 2653
  • [2] DETECTION OF CHLAMYDIA-TRACHOMATIS IN URINE SPECIMENS FROM WOMEN BY LIGASE CHAIN-REACTION
    BASSIRI, M
    HU, HY
    DOMEIKA, MA
    BURCZAK, J
    SVENSSON, LO
    LEE, HH
    MARDH, PA
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) : 898 - 900
  • [3] *BUND GES, 1996, UB MELD INF RANKH SC, P13
  • [4] CATRY MA, 1995, GENITOURIN MED, V71, P247
  • [5] DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS INFECTIONS IN MEN AND WOMEN BY TESTING FIRST-VOID URINE BY LIGASE CHAIN-REACTION
    CHERNESKY, MA
    JANG, D
    LEE, H
    BURCZAK, JD
    HU, H
    SELLORS, J
    TOMAZICALLEN, SJ
    MAHONY, JB
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (11) : 2682 - 2685
  • [6] DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS URETHRAL INFECTION IN SYMPTOMATIC AND ASYMPTOMATIC MEN BY TESTING FIRST-VOID URINE IN A LIGASE CHAIN-REACTION ASSAY
    CHERNESKY, MA
    LEE, H
    SCHACHTER, J
    BURCZAK, JD
    STAMM, WE
    MCCORMACK, WM
    QUINN, TC
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (05) : 1308 - 1311
  • [7] LIGASE CHAIN-REACTION FOR DETECTION OF NEISSERIA-GONORRHOEAE IN UROGENITAL SWABS
    CHING, SF
    LEE, H
    HOOK, EW
    JACOBS, MR
    ZENILMAN, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (12) : 3111 - 3114
  • [8] COUTINHO RA, 1994, SEX TRANSM DIS S2, V21, P1
  • [9] AMPLIFICATION OF CHLAMYDIA-TRACHOMATIS DNA BY LIGASE CHAIN-REACTION
    DILLE, BJ
    BUTZEN, CC
    BIRKENMEYER, LG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) : 729 - 731
  • [10] DIRENZO S, 1995, 95 GEN M AM SOC MICR, P86