Regulation of stress response in Oenococcus oeni as a function of environmental changes and growth phase

被引:71
作者
Guzzo, J
Jobin, MP
Delmas, F
Fortier, LC
Garmyn, D
Tourdot-Maréchal, R
Lee, B
Divies, C
机构
[1] Univ Bourgogne 1, Ecole Natl Super Biol Appl Nutr & Alimentat, Microbiol Lab UA, INRA, F-21000 Dijon, France
[2] McGill Univ, Fac Agr & Environm Sci, Dept Food Sci & Agr Chem, Ste Anne De Bellevue, PQ H9X 3V9, Canada
关键词
Oenococcus; malolactic fermentation; stress gene; ATPase;
D O I
10.1016/S0168-1605(00)00209-9
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Oenococcus oeni is a lactic acid bacterium which is able to grow in wine and perform malolactic fermentation. To survive and grow in such a harsh environment as wine, O. oeni uses several mechanisms of resistance including stress protein synthesis. The molecular characterisation of three stress genes hsp-18, clpX, trxA encoding for a small heat shock protein, an ATPase regulation component of ClpP protease and a thioredoxin, respectively, allow us to suggest the existence in O. oeni of multiple regulation mechanisms as is the case in Bacillus subtilis. One common feature of these genes is that they are expressed under the control of housekeeping promoters. The expression of these genes as a function of growth is significantly different. Surprisingly, the clpX gene, which is induced by heat shock, was highly expressed in the early phase of growth. In addition to stress protein synthesis, adaptation to the acid pH of wine requires efficient cellular systems to extrude protons. Using inhibitors specific for different types of ATPases, we demonstrated the existence of H+-ATPase and P-type ATPase. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:27 / 31
页数:5
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