Orthogonal (transverse) arrangements of actin in endothelia and fibroblasts

被引:7
作者
Curtis, Adam [1 ]
Aitchison, Gregor
Tsapikouni, Theodora
机构
[1] Univ Glasgow, Ctr Cell Engn, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Patras, Dept Engn Mech, GR-26500 Patras, Greece
关键词
transverse (orthogonal) actin; polarization microscopy; force measurement;
D O I
10.1098/rsif.2006.0132
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Though actin. laments running across the cell (transverse actin) have been occasionally reported for epithelial cells in groups and for cells growing on fibres, there has been no report heretofore of transverse actin in cells grown on planar substrata. This paper describes evidence in support of this possibility derived from actin staining, polarization microscopy and force measurements. The paper introduces two new methods for detecting the orientation and activity of contractile elements in cells. The orthogonal actin is most obvious in cells grown on groove ridge structures, but can be detected in cells grown on. at surfaces.
引用
收藏
页码:753 / 756
页数:4
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