Full characterization of PDX, a neuroprotectin/protectin D1 isomer, which inhibits blood platelet aggregation

被引：85

作者：

Chen, P. ^{[1
]}

Fenet, B. ^{[2
]}

Michaud, S. ^{[1
]}

Tomczyk, N. ^{[3
]}

Vericel, E. ^{[1
]}

Lagarde, M. ^{[1
]}

Guichardant, M. ^{[1
]}

机构：

[1] Univ Lyon, Insa Lyon, RMND, IMBL,Inserm,UMR 870,Inra 1235, F-69621 Villeurbanne, France

[2] UCB Lyon 1, ESCPE, CCRMN, Lyon, France

[3] Waters Corp, Manchester M22 5PP, Lancs, England

来源：

FEBS LETTERS | 2009年 / 583卷 / 21期

关键词：

Docosahexaenoic acid; 15-Lipoxygenase; 10,17-Dihydroxy-docosahexaenoic acid; Double lipoxygenation; Ion mobility separation; DOCOSAHEXAENOIC ACID; OXIDATIVE STRESS; ARACHIDONIC-ACID; BRAIN; LEUKOCYTES; CELLS; DOCOSATRIENES; BIOSYNTHESIS; LIPOXYGENASE; LEUKOTRIENES;

D O I：

10.1016/j.febslet.2009.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our study aimed to establish the complete structure of the main dihydroxy conjugated triene issued from the lipoxygenation (soybean enzyme) of docosahexaenoic acid, named PDX, an isomer of protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches and other chemical characterization (e. g. GC-MS, HPLC and LC-MS/MS) indicated that PDX is 10(S), 17(S)-dihydroxydocosahexa-4Z,7Z, 11E, 13Z, 15E,19Z-enoic acid. The use of O-18(2) and mass spectrometry showed that PDX is a double lipoxygenation product. Its structure differs from PD1, with E, Z, E geometry (PDX) instead of E, E, Z (PD1) and S configuration at carbon 10 instead of R. PDX inhibits human blood platelet aggregation at sub-micromolar concentrations. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3478 / 3484

页数：7

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