Construction of Langmuir-Blodgett films from light-harvesting complex I isolated from photosynthetic bacteria

被引：9

作者：

Iida, K

Kashiwada, A

Nango, M

机构：

[1] Nagoya Municipal Ind Res Inst, Atsuta Ku, Nagoya, Aichi 4560058, Japan

[2] Nagoya Inst Technol, Dept Appl Chem, Showa Ku, Nagoya, Aichi 4668555, Japan

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2000年 / 169卷 / 1-3期

关键词：

light-harvesting complex; Langmuir-Blodgett film; membrane materials; bacteriochlorophyll a;

D O I：

10.1016/S0927-7757(00)00436-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Langmuir-Blodgett (LB) films of the light-harvesting (LH) polypeptides/bacteriochlorophyll a (BChl a) complex isolated from photosynthetic bacteria, Rhodospirillum rubrum, were constructed. The monolayers of the core LH /BChl a complex were prepared from octyl beta-glycoside suspensions at an air/water interface and then the monolayer was transferred to glass slides using LB technique multilayer films. UV-vis absorption spectra of the LB layer films absorbing 870 nm at the Qy absorption of BChl a indicated that the LH/BChl a complex in the multilayer films was more stable than the subunit complex absorbing 820 nm at the Qy absorption. Analysis of Fourier transform infrared reflection absorption spectroscopy (FTIR-RAS) spectra showed that the tilt angle of the transmembrane axis of the LH/BChl a complex in the multilayer films was 40 degrees to the normal. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：199 / 208

页数：10

共 41 条

[1] PRESSURE-INDUCED CHANGES IN THE ABSORPTION-SPECTRUM OF MONOLAYERS AT THE AIR-WATER-INTERFACE - COMPARISON OF CALCULATIONS WITH EXPERIMENTS [J].