Intralobular ducts of human major salivary glands contain leptin and its receptor

被引:44
作者
De Matteis, R
Puxeddu, R
Riva, A
Cinti, S
机构
[1] Univ Ancona, Inst Normal Human Morphol Anat, I-60020 Ancona, Italy
[2] Univ Cagliari, Dept Surg Sci & Organ Transplantat, I-09124 Cagliari, Italy
[3] Univ Cagliari, Dept Cytomorphol, I-09124 Cagliari, Italy
关键词
glucocorticoid receptor; human salivary gland; immunohistochemistry; leptin;
D O I
10.1046/j.0021-8782.2002.00106.x
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Leptin, a 16-kDa hormone, plays an important role in the control of food intake and in energy homeostasis both in rodents and in man. Leptin is mainly produced and secreted by adipocytes, but other tissues and gastric glands have also recently been shown to produce it in a dual (endocrine and exocrine) mode. In addition, a leptin receptor has been detected in taste cells of mouse circumvallate papillae and in rat intestinal epithelium. These data prompted us to carry out a detailed study of human salivary glands as potential leptin-producing organs. Biopsies of salivary glands (submandibular and parotid) obtained from male and female patients during surgery for different clinical indications were subjected to immunohistochemical study for the presence of leptin, its functional receptor, insulin and glucagon. The presence and cellular distribution of glucocorticoid receptor in leptin-secreting cells were also investigated. Double immunohistochemical staining (silver-gold intensification and avid in-biotin-peroxidase) was used for the visualization of glucocorticoid receptor and leptin labelling, respectively. The results show that intralobular duct cells of submanclibular and parotid glands are immunoreactive for leptin, leptin receptor and glucagon but not for insulin. Leptin was also detected in some microglobules in whole saliva obtained from four healthy volunteers. Co-localization for leptin, leptin receptor and glucocorticoid receptor in the same cell type suggested a functional relationship between glucocorticoid hormone and leptin secretion also at the level of the salivary glands. Key words glucocorticoid receptor; human salivary gland; immunohistochemistry; leptin.
引用
收藏
页码:363 / 370
页数:8
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