Direct electrochemical redox of tyrosinase at silver electrodes

被引:18
作者
Ye, BX [1 ]
Zhou, XY [1 ]
机构
[1] WUHAN UNIV,DEPT CHEM,WUHAN 430072,PEOPLES R CHINA
基金
中国国家自然科学基金;
关键词
direct electrochemistry; silver electrode; tyrosinase;
D O I
10.1016/S0039-9140(96)02121-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The direct electron transfer reactions between tyrosinase and silver electrode were investigated by using cyclic voltammetry and potential-step chronoamperometry as well as current-step chronopotentiometry techniques. The kinetics of these reactions is quasi-reversible with two electron transfer reactions and 0.030 s(-1) apparent electrode reaction rate constant. The results demonstrate that neither electrode surface modification nor the inclusion of mediators is necessary to study the electron transfer reactions of tyrosinase at silver electrodes. Moreover, both the anodic and the cathodic currents are linear relationship with the tyrosinase concentration in the rang of 1 x 10(-9) similar to x 10(-8) mol l(-1). It is possible to be used as a method of analyzing tyrosinase concentration. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:831 / 836
页数:6
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