Conversion of sucrose into isomaltulose by Enterobacter sp FMB1, an isomaltulose-producing microorganism isolated from traditional Korean food

被引:37
作者
Cho, Mee-Hyun
Park, Sang-Eun
Lim, Jin Kyu
Kim, Jong-Sang
Kim, Jeong Hwan
Kwon, Dae Young
Park, Cheon-Seok [1 ]
机构
[1] Kyung Hee Univ, Dept Food Sci & Biotechnol, Grad Sch Biotechnol, Yongin 449701, South Korea
[2] Kyung Hee Univ, Inst Life Sci & Resources, Yongin 449701, South Korea
[3] Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Taegu 702701, South Korea
[4] Gyeongsang Natl Univ, Dept Food Sci & Technol, Jinju 660701, South Korea
[5] Korea Food Res Inst, Songnam 463746, South Korea
关键词
isomaltulose; Maeju; Nuruk; sucrose isomerase; trehalulose;
D O I
10.1007/s10529-006-9257-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Over 500 microorganisms isolated from Korean traditional foods, Maeju (source of soybean paste) and Nuruk (Korean koji), were screened to obtain an isomaltulose-producing microorganism. It was identified as Enterobacter sp. FMB-1 by 16S rRNA sequencing and the API 20E system. It had a greater than 90% conversion of sucrose (as 4 g/l) to isomaltulose in 2 days. Small amounts of trehalulose, glucose, and fructose were produced as byproducts, implying that this strain could be possibly employed in the production of isomaltulose in industry.
引用
收藏
页码:453 / 458
页数:6
相关论文
共 21 条
[1]   Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici [J].
Ahn, SJ ;
Yoo, JH ;
Lee, HC ;
Kim, JH ;
Lee, JK .
BIOTECHNOLOGY LETTERS, 2003, 25 (14) :1179-1183
[2]  
BAER A, 1989, LEBENSM WISS TECHNOL, V22, P46
[3]   Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici [J].
Börnke, F ;
Hajirezaei, M ;
Sonnewald, U .
JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2425-2430
[4]   Conversion of sucrose to isomaltulose by Klebsiella planticola CCRC 19112 [J].
Huang, JH ;
Hsu, LH ;
Su, YC .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 21 (1-2) :22-27
[5]  
Kashimura J, 2003, J NUTR SCI VITAMINOL, V49, P214, DOI 10.3177/jnsv.49.214
[6]  
KAWAI K, 1985, ENDOCRINOL JAPON, V32, P933
[7]   Substrate induction of isomaltulose synthase in a newly isolated Klebsiella sp LX3 [J].
Li, X ;
Zhao, C ;
An, Q ;
Zhang, D .
JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (03) :521-527
[8]   Isomaltulose (Palatinose®):: a review of biological and toxicological studies [J].
Lina, BAR ;
Jonker, D ;
Kozianowski, G .
FOOD AND CHEMICAL TOXICOLOGY, 2002, 40 (10) :1375-1381
[9]  
MACALLISTER M, 1990, BIOTECHNOL LETT, V12, P667
[10]  
Matsukubo T, 2006, INT DENT J, V56, P119