Changes in gene expression pattern following granulocyte colony-stimulating factor administration to normal stem cell sibling donors

被引:23
作者
Amariglio, Ninette
Jacob-Hirsch, Jasmine
Shimoni, Avichai
Leiba, Merav
Rechavi, Gideon
Nagler, Arnon [1 ]
机构
[1] Chaim Sheba Med Ctr, Div Hematol, IL-52621 Tel Hashomer, Israel
[2] Chaim Sheba Med Ctr, Sheba Canc Res Ctr, IL-52621 Tel Hashomer, Israel
关键词
granulocyte colony-stimulating factor; gene chip array; expression level; normal donors; stem cell transplantation;
D O I
10.1159/000096856
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Granulocyte colony-stimulating factor (G-CSF) is widely used for the mobilization of hematopoietic stem cells from normal donors. The mechanism of induced mobilization has recently been elucidated. Treatment with G-CSF is considered safe; however, long-term effects are largely unknown. The aim of this study was, therefore, to determine whether it leads to significant changes in gene expression modifications. Affymetrix Gene Chip array technology was used. Samples were collected before and at various time points (up to 9 months) after mobilization. The expression levels of 122 genes were transiently modified before and after mobilization (p < 0.05). Fifty-three genes belonging to cell growth, proliferation and communication gene ontology categories were upregulated, while 69 genes were downregulated. Conclusion: The administration of G-CSF is associated with transient DNA and gene expression modifications in the lymphocytes of normal mobilized donors. A long-term follow-up of stem cell donors is recommended. Copyright (c) 2007 S. Karger AG, Basel.
引用
收藏
页码:68 / 73
页数:6
相关论文
共 14 条
  • [1] Biologic and clinical effects of granulocyte colony-stimulating factor in normal individuals
    Anderlini, P
    Przepiorka, D
    Champlin, R
    Korbling, M
    [J]. BLOOD, 1996, 88 (08) : 2819 - 2825
  • [2] Severe chronic neutropenia: Treatment and follow-up of patients in the severe chronic neutropenia international registry
    Dale, DC
    Cottle, TE
    Fier, CJ
    Bolyard, AA
    Bonilla, MA
    Boxer, LA
    Cham, B
    Freedman, MH
    Kannourakis, G
    Kinsey, SE
    Davis, R
    Scarlata, D
    Schwinzer, B
    Zeidler, C
    Welte, K
    [J]. AMERICAN JOURNAL OF HEMATOLOGY, 2003, 72 (02) : 82 - 93
  • [3] Expression profiling and functional analysis of Wnt signaling mechanisms in mesenchymal stem cells
    Etheridge, SL
    Spencer, GJ
    Heath, DJ
    Genever, PG
    [J]. STEM CELLS, 2004, 22 (05) : 849 - 860
  • [4] Freedman MH, 2000, BLOOD, V96, P429
  • [5] Neutrophil specific granule deficiency and mutations in the gene encoding transcription factor C/EBPε
    Gombart, AF
    Koeffler, HP
    [J]. CURRENT OPINION IN HEMATOLOGY, 2002, 9 (01) : 36 - 42
  • [6] Regulation of neutrophil and eosinophil secondary granule gene express-ion by transcription factors C/EBPε and PU.1
    Gombart, AF
    Kwok, SH
    Anderson, KL
    Yamaguchi, Y
    Torbett, BE
    Koeffler, HP
    [J]. BLOOD, 2003, 101 (08) : 3265 - 3273
  • [7] Mobilisation with G-CSF in healthy donors promotes a high but temporal deregulation of genes
    Hernández, JM
    Castilla, C
    Gutiérrez, NC
    Isidro, IM
    Delgado, M
    de las Rivas, J
    Fermiñán, E
    García, JL
    Ocio, EM
    del Cañizo, MC
    San Miguel, JF
    [J]. LEUKEMIA, 2005, 19 (06) : 1088 - 1091
  • [8] Granulocyte colony-stimulating factor generates epigenetic and genetic alterations in lymphocytes of normal volunteer donors of stem cells
    Nagler, A
    Korenstein-Ilan, A
    Amiel, A
    Avivi, L
    [J]. EXPERIMENTAL HEMATOLOGY, 2004, 32 (01) : 122 - 130
  • [9] G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4
    Petit, I
    Szyper-Kravitz, M
    Nagler, A
    Lahav, M
    Peled, A
    Habler, L
    Ponomaryov, T
    Taichman, RS
    Arenzana-Seisdedos, F
    Fujii, N
    Sandbank, J
    Zipori, D
    Lapidot, T
    [J]. NATURE IMMUNOLOGY, 2002, 3 (07) : 687 - 694
  • [10] Weighing the risks of G-CSF administration, leukopheresis, and standard marrow harvest: Ethical and safety considerations for normal pediatric hematopoietic cell donors
    Pulsipher, MA
    Nagler, A
    Iannone, R
    Nelson, RM
    [J]. PEDIATRIC BLOOD & CANCER, 2006, 46 (04) : 422 - 433