Arabidopsis thaliana mutants altered in homologous recombination

被引:40
作者
Masson, JE
Paszkowski, J
机构
[1] Friedrich Miescher Institute, 4002 Basel
关键词
D O I
10.1073/pnas.94.21.11731
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Homologous recombination contributes both to the generation of allelic diversity and to the preservation of genetic information, In plants, a lack of suitable experimental material has prevented studies of the regulatory and enzymatic aspects of recombination in somatic and meiotic cells. We have isolated nine Arabidopsis thaliana mutants hypersensitive to x-ray irradiation (xrs) and examined their recombination properties. For the three xrs loci described here, single recessive mutations were found to confer simultaneous hypersensitivities to the DNA-damaging chemicals mitomycin C (MMCs) and/or methyl methanesulfonate (MMSs) and alterations in homologous recombination. Mutant xrs9 (Xray(s), MMSs) is reduced in both somatic and meiotic recombination and resembles yeast mutants of the rad52 epistatic group. xrs11 (Xray(s), MMCs) is deficient in the x-ray-mediated stimulation of homologous recombination in somatic cells in a manner suggesting a specific signaling defect. xrs4 (Xray(s), MMSs, MMCs) has a significant deficiency in somatic recombination, but this is accompanied by meiotic hyper-recombination, A corresponding phenotype has not been reported in other systems and thus this indicates a novel, plant-specific regulatory circuit linking mitotic and meiotic recombination.
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页码:11731 / 11735
页数:5
相关论文
共 37 条
[1]  
BAKER BS, 1976, ANNU REV GENET, V10, P109
[2]   SINGLE-STRANDED-DNA AS A RECOMBINATION SUBSTRATE IN PLANTS AS ASSESSED BY STABLE AND TRANSIENT RECOMBINATION ASSAYS [J].
BILANG, R ;
PETERHANS, A ;
BOGUCKI, A ;
PASZKOWSKI, J .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (01) :329-336
[3]  
BURT A, 1991, J EVOLUTION BIOL, V4, P887
[4]   A GENETIC-BASIS FOR VARIATIONS IN MEIOTIC RECOMBINATION IN PETUNIA-HYBRIDA [J].
CORNU, A ;
FARCY, E ;
MOUSSET, C .
GENOME, 1989, 32 (01) :46-53
[5]   A SOMATIC GENE REARRANGEMENT CONTRIBUTING TO GENETIC DIVERSITY IN MAIZE [J].
DAS, OP ;
LEVIMINZI, S ;
KOURY, M ;
BENNER, M ;
MESSING, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :7809-7813
[6]  
DEVEAUX LC, 1992, GENETICS, V130, P251
[7]  
Ganal MW, 1996, THEOR APPL GENET, V92, P101, DOI 10.1007/BF00222958
[8]   HRR25, A PUTATIVE PROTEIN-KINASE FROM BUDDING YEAST - ASSOCIATION WITH REPAIR OF DAMAGED DNA [J].
HOEKSTRA, MF ;
LISKAY, RM ;
OU, AC ;
DEMAGGIO, AJ ;
BURBEE, DG ;
HEFFRON, F .
SCIENCE, 1991, 253 (5023) :1031-1034
[9]   HIGH-FIDELITY EXTRACHROMOSOMAL RECOMBINATION AND GENE TARGETING IN PLANTS [J].
HROUDA, M ;
PASZKOWSKI, J .
MOLECULAR AND GENERAL GENETICS, 1994, 243 (01) :106-111
[10]   MUTATION OF THE GENE ENCODING PROTEIN-KINASE C1 STIMULATES MITOTIC RECOMBINATION IN SACCHAROMYCES-CEREVISIAE [J].
HUANG, KN ;
SYMINGTON, LS .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (09) :6039-6045