Regulation of pollen tube growth by Rac-like GTPases

被引:66
作者
Cheung, AY [1 ]
Chen, CYH
Tao, LZ
Andreyeva, T
Twell, D
Wu, HM
机构
[1] Univ Massachusetts, Dept Biochem & Mol Biol, LGRT, Amherst, MA 01003 USA
[2] Univ Massachusetts, Cell & Mol Biol Program, LGRT, Amherst, MA 01003 USA
[3] Univ Massachusetts, Plant Biol Grad Program, LGRT, Amherst, MA 01003 USA
[4] Univ Leicester, Dept Biol, Leicester LE1 7RA, Leics, England
关键词
pollen tube; profiling of pollen Rac GTPases; signalling of polar growth;
D O I
10.1093/jxb/54.380.73
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant Rac-like GTPases have been classified phylogenetically into two major groups-class I and class II. Several pollen-expressed class I Rac-like GTPases have been shown to be important regulators of polar pollen tube growth. The functional participation by some of the class I and all of the class II Arabidopsis Rac-like GTPases in pollen tube growth remains to be explored. It is shown that at least four members of the Arabidopsis Rac GTPase family are expressed in pollen, including a class II Rac, AtRac7. However, when over-expressed as fusion proteins with GFP, both pollen- and non-pollen-expressed AtRacs interfered with the normal pollen tube tip growth process. These observations suggest that these AtRacs share similar biochemical activities and may integrate into the pollen cellular machinery that regulates the polar tube growth process. Therefore, the functional contribution by individual Rac GTPase to the pollen tube growth process probably depends to a considerable extent on their expression characteristics in pollen. Among the Arabidopsis Racs, GFP-AtRac7 showed association with the cell membrane and Golgi bodies, a pattern distinct from all previously reported localization for other plant Racs. Over-expressing GFP-AtRac7 also induced the broadest spectrum of pollen tube growth defects, including pollen tubes that are bifurcated, with diverted growth trajectory or a ballooned tip. Transgenic plants with multiple copies of the chimeric Lat52-GFP-AtRac7 showed severely reduced seed set, probably many of these defective pollen tubes were arrested, or reduced in their growth rates that they did not arrive at the ovules while they were still receptive for fertilization. These observations substantiate the importance of Rac-like GTPases to sexual reproduction.
引用
收藏
页码:73 / 81
页数:9
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