Programmed cell death induced by (β-D-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells

被引：47

作者：

Chaves, I

Regalado, AP

Chen, M

Ricardo, CP

Showalter, AM

机构：

[1] Inst Tecnol Quim & Biol, Plant Biochem Lab, P-2780901 Oeiras, Portugal

[2] Ohio Univ, Dept Environm & Plant Biol, Mol & Cellular Biol Program, Athens, OH 45701 USA

[3] Inst Super Agron, P-1349017 Lisbon, Portugal

来源：

PHYSIOLOGIA PLANTARUM | 2002年 / 116卷 / 04期

关键词：

D O I：

10.1034/j.1399-3054.2002.1160414.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Programmed cell death (PCD) is involved in plant development and pathogen defence and can be triggered in vitro by several biotic and abiotic stimuli. In this report (beta-D-galactosyl)(3) Yariv reagent, a chemical that specifically binds to arabinogalactan-proteins (AGPs), completely inhibited cell growth and induced PCD in tobacco BY-2 suspension cultured cells. Analysis of DNA from these cells, by agarose gel electrophoresis, revealed a DNA ladder consisting of multimers of 140-170 bp, similar to apoptotic animal DNA internucleosomal fragmentation. Complementary morphological studies revealed additional PCD characteristics in the Yariv-treated BY-2 cells, including cell shrinkage and cytoplasmic condensation. These studies demonstrate the usefulness of BY-2 cells as a model plant PCD system and confirm a link between AGPs and PCD.

引用

页码：548 / 553

页数：6

共 23 条

[1] Prediction of glycosylphosphatidylinositol-anchored proteins in arabidopsis. A genomic analysis [J].