Long and short forms of the ovine prolactin receptor: cDNA cloning and genomic analysis reveal that the two forms arise by different alternative splicing mechanisms in ruminants and in rodents

被引:74
作者
Bignon, C
Binart, N
Ormandy, C
Schuler, LA
Kelly, PA
Djiane, J
机构
[1] FAC MED NECKER ENFANTS MALAD, INSERM, U344, F-75730 PARIS 15, FRANCE
[2] UNIV WISCONSIN, DEPT COMPARAT BIOSCI, MADISON, WI 53706 USA
关键词
D O I
10.1677/jme.0.0190109
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Prolactin is a pituitary hormone that binds to specific receptors in numerous tissues. Depending on the size of their cytoplasmic domain, long and short prolactin receptors (l-PR, s-PR) have been described. Up to now, s-PR were found in rodents only. We report here the cloning of full-length coding sequences for short and long ovine prolactin receptors (s-oPR, l-oPR). The only difference between s- and l-oPR coding sequences was, respectively, the presence or absence of a 39 base pair insert at the beginning of the cytoplasmic domain, with two contiguous inframe stop codons at its 3' end. Sequence comparison revealed that the alternative splicing producing s- and l-oPR was different from that of rodents, although the resulting proteins were very similar. PCR experiments on ovine genomic DNA showed that the 39 base pair insert was directly linked to the downstream exon, and separated from the upstream exon by an 800 base pair intron. Thus, the alternative splicing used a single intron with one 5' and two 3' sites. The same organization was found in bovine and caprine genomes, suggesting that this feature is general in ruminants and different from rodents, which use mutually exclusive exons to produce s-PR and l-PR.
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页码:109 / 120
页数:12
相关论文
共 24 条
[1]   2 FORMS OF THE PROLACTIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID ARE PRESENT IN OVINE FETAL LIVER AND ADULT OVARY [J].
ANTHONY, RV ;
SMITH, GW ;
DUONG, A ;
PRATT, SL ;
SMITH, MF .
ENDOCRINE, 1995, 3 (04) :291-295
[2]   CLONING AND EXPRESSION OF THE RAT PROLACTIN RECEPTOR, A MEMBER OF THE GROWTH-HORMONE PROLACTIN RECEPTOR GENE FAMILY [J].
BOUTIN, JM ;
JOLICOEUR, C ;
OKAMURA, H ;
GAGNON, J ;
EDERY, M ;
SHIROTA, M ;
BANVILLE, D ;
DUSANTERFOURT, I ;
DJIANE, J ;
KELLY, PA .
CELL, 1988, 53 (01) :69-77
[3]   IDENTIFICATION OF A CDNA-ENCODING A LONG FORM OF PROLACTIN RECEPTOR IN HUMAN HEPATOMA AND BREAST-CANCER CELLS [J].
BOUTIN, JM ;
EDERY, M ;
SHIROTA, M ;
JOLICOEUR, C ;
LESUEUR, L ;
ALI, S ;
GOULD, D ;
DJIANE, J ;
KELLY, PA .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (09) :1455-1461
[4]   MULTIPLE FORMS OF PROLACTIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID ARE SPECIFICALLY EXPRESSED AND REGULATED IN MURINE TISSUES AND THE MAMMARY CELL LINE-HC11 [J].
BUCK, K ;
VANEK, M ;
GRONER, B ;
BALL, RK .
ENDOCRINOLOGY, 1992, 130 (03) :1108-1114
[5]   TRANSDUCTION OF PROLACTINS (PRL) GROWTH SIGNAL THROUGH BOTH LONG AND SHORT FORMS OF THE PRL RECEPTOR [J].
DAS, R ;
VONDERHAAR, BK .
MOLECULAR ENDOCRINOLOGY, 1995, 9 (12) :1750-1759
[6]   EXPRESSION OF MULTIPLE FORMS OF THE PROLACTIN RECEPTOR IN MOUSE-LIVER [J].
DAVIS, JA ;
LINZER, DIH .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (04) :674-680
[7]   PROLACTIN RECEPTOR GENE-EXPRESSION IN THE RABBIT - IDENTIFICATION, CHARACTERIZATION AND TISSUE DISTRIBUTION OF SEVERAL PROLACTIN RECEPTOR MESSENGER-RNAS ENCODING A UNIQUE PRECURSOR [J].
DUSANTERFOURT, I ;
GAYE, P ;
BELAIR, L ;
PETRIDOU, B ;
KELLY, PA ;
DJIANE, J .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1991, 77 (1-3) :181-192
[8]   A LIMITED CYTOPLASMIC REGION OF THE PROLACTIN RECEPTOR CRITICAL FOR SIGNAL-TRANSDUCTION [J].
EDERY, M ;
LEVIMEYRUEIS, C ;
PALY, J ;
KELLY, PA ;
DJIANE, J .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1994, 102 (1-2) :39-44
[9]   IDENTIFICATION AND SEQUENCE-ANALYSIS OF A 2ND FORM OF PROLACTIN RECEPTOR BY MOLECULAR-CLONING OF COMPLEMENTARY-DNA FROM RABBIT MAMMARY-GLAND [J].
EDERY, M ;
JOLICOEUR, C ;
LEVIMEYRUEIS, C ;
DUSANTERFOURT, I ;
PETRIDOU, B ;
BOUTIN, JM ;
LESUEUR, L ;
KELLY, PA ;
DJIANE, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (06) :2112-2116
[10]   PROLACTIN RECEPTOR ANTAGONISTS THAT INHIBIT THE GROWTH OF BREAST-CANCER CELL-LINES [J].
FUH, G ;
WELLS, JA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (22) :13133-13137