Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family

被引：87

作者：

Iwamoto, Tomotada ^{[1
]}

Yoshida, Shiomi

Suzuki, Katsuhiro

Tomita, Motohisa

Fujiyama, Riyo

Tanaka, Noriko

Kawakami, Yasuto

Ito, Masahiro

机构：

[1] Kobe Inst Hlth, Dept Microbiol, Kobe, Hyogo, Japan

[2] Natl Hosp Org, Kinki Chuo Chest Med Ctr, Osaka, Japan

[3] Kobe City Publ Hlth Ctr, Kobe, Hyogo, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 270卷 / 01期

关键词：

Mycobacterium tuberculosis Beijing family; VNTR; tuberculosis; IS6110; RFLP;

D O I：

10.1111/j.1574-6968.2007.00658.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The newly proposed 15- and 24-loci mycobacterial interspersed repetitive unit (MIRU)-variable-number tandem repeat (VNTR) typing method was evaluated for its ability to differentiate 181 Mycobacterium tuberculosis Beijing family strains. Compared with the original 12-loci MIRU-VNTR typing method, the 15-loci system dramatically improved the discriminatory power for Beijing strains; however, large clusters that could be further differentiated by IS6110 restriction fragment length polymorphism (RFLP) were still obtained. The clonal stability and allelic diversity of a total of 31 VNTR loci were evaluated. VNTRs 3232, 3820, and 4120 were identified as the effective hypervariable VNTR set for the second-line typing of clustered strains following the 15-loci based scheme. Consequently, the discriminatory power of the new scheme (18 loci) equaled that of IS6110 RFLP.

引用

页码：67 / 74

页数：8

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