Protein array autoantibody profiles for insights into systemic lupus erythematosus and incomplete lupus syndromes

被引:153
作者
Li, Q. -Z.
Zhou, J.
Wandstrat, A. E.
Carr-Johnson, F.
Branch, V.
Karp, D. R.
Mohan, C.
Wakeland, E. K.
Olsen, N. J.
机构
[1] Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr, Dept Med, Div Rheumat Dis, Dallas, TX 75390 USA
关键词
autoantibodies; autoantigens; autoimmunity; incomplete lupus; proteome microarray; systemic lupus erythematosus;
D O I
10.1111/j.1365-2249.2006.03251.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The objective of this study was to investigate the prevalence and clinical significance of a spectrum of autoantibodies in systemic lupus erythernatosus and incomplete lupus syndromes using a proteome microarray bearing 70 autoantigens. Microarrays containing candidate autoantigens or control proteins were printed on 16-section slides. These arrays were used to profile 93 serum samples from patients with systemic lupus erythernatosus (SLE (n = 33), incomplete LE (ILE; n = 23), first-degree relatives (FDRs) of SLE patients (n = 20) and non-autoimmune controls (NC; n = 17). Data were analysed using the significance analysis of microarray (SAM) and clustering algorithms. Correlations with disease features were determined. Serum from ILE and SLE patients contained high levels of IgG autoantibodies to 50 autoantigens and IgM autoantibodies to 12 autoantigens. Elevated levels of at least one IgG autoantibody were detected in 26% of SLE and 19% of ILE samples; elevated IgM autoantibodies were present in 13% of SLE and 17% of ILE samples. IgG autoantibodies segregated into seven clusters including two specific for DNA and RNA autoantigens that were correlated with the number of lupus criteria. Three IgG autoantibody clusters specific for collagens, DNA and histones, were correlated with renal involvement. Of the four IgM autoantibody clusters, two were correlated negatively with the number of lupus criteria; none were correlated with renal disease. The IgG : IgM autoantibody ratios generally showed a stepwise increase in the groups following disease burden from NC to SLE. Insights derived from the expanded autoantibody profiling made possible with the antigen array suggest differences in autoreactivity in ILE and SLE. Determining whether the IgM aurotreactivity that predominates in ILE represents an early stage prior to IgG switching or is persistent and relatively protective will require further longitudinal studies.
引用
收藏
页码:60 / 70
页数:11
相关论文
共 32 条
[1]   Familial aggregation of systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases in 1,177 lupus patients from the GLADEL cohort [J].
Alarcon-Segovia, D ;
Alarcón-Riquelme, ME ;
Cardiel, MH ;
Caeiro, F ;
Massardo, L ;
Villa, AR ;
Pons-Estel, BA .
ARTHRITIS AND RHEUMATISM, 2005, 52 (04) :1138-1147
[2]   Development of autoantibodies before the clinical onset of systemic lupus erythematosus [J].
Arbuckle, MR ;
McClain, MT ;
Rubertone, MV ;
Scofield, RH ;
Dennis, GJ ;
James, JA ;
Harley, JB .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (16) :1526-1533
[3]   Clinical presentation and monitoring of lupus nephritis [J].
Balow, JE .
LUPUS, 2005, 14 (01) :25-30
[4]   Anti-chromatin antibodies in systemic lupus erythematosus:: a useful marker for lupus nephropathy [J].
Cervera, R ;
Viñas, O ;
Ramos-Casals, M ;
Font, J ;
García-Carrasco, M ;
Sisó, A ;
Ramírez, F ;
Machuca, Y ;
Vives, J ;
Ingelmo, M ;
Burlingame, RW .
ANNALS OF THE RHEUMATIC DISEASES, 2003, 62 (05) :431-434
[5]   ANTIBODIES TO HISTONES IN SYSTEMIC LUPUS-ERYTHEMATOSUS - PREVALENCE, SPECIFICITY, AND RELATIONSHIP TO CLINICAL AND LABORATORY FEATURES [J].
COHEN, MG ;
POLLARD, KM ;
WEBB, J .
ANNALS OF THE RHEUMATIC DISEASES, 1992, 51 (01) :61-66
[6]   Antihistone and anti-double-stranded deoxyribonucleic acid antibodies are associated with renal disease in systemic lupus erythematosus [J].
Cortés-Hernández, J ;
Ordi-Ros, J ;
Labrador, M ;
Buján, S ;
Balada, E ;
Segarra, A ;
Vilardell-Tarrés, M .
AMERICAN JOURNAL OF MEDICINE, 2004, 116 (03) :165-173
[7]   The use of laboratory tests in the diagnosis of SLE [J].
Egner, W .
JOURNAL OF CLINICAL PATHOLOGY, 2000, 53 (06) :424-432
[8]   Outcome of incomplete systemic lupus erythematosus after 10 years [J].
Hallengren, CS ;
Nived, O ;
Sturfelt, G .
LUPUS, 2004, 13 (02) :85-88
[9]   Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus [J].
Hochberg, MC .
ARTHRITIS AND RHEUMATISM, 1997, 40 (09) :1725-1725
[10]  
HOCHBERG MC, 2001, EPIDEMIOLOGY RHEUMAT, P123