Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus

被引:106
作者
Cheung, Johnson [1 ]
Beasley, Federico C. [1 ]
Liu, Suya [2 ]
Lajoie, Gilles A. [2 ]
Heinrichs, David E. [1 ]
机构
[1] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5C1, Canada
[2] Univ Western Ontario, Dept Biochem, London, ON N6A 5C1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
SIDEROPHORE BIOSYNTHESIS; IRON ACQUISITION; GENE-CLUSTER; CITRIC-ACID; IDENTIFICATION; PATHWAY; CONDENSATION; SYNTHETASES; SPERMIDINE; REGULATOR;
D O I
10.1111/j.1365-2958.2009.06880.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
P>Siderophores are iron-scavenging molecules produced by many microbes. In general, they are synthesized using either non-ribosomal peptide synthetase (NRPS) or NRPS-independent siderophore (NIS) pathways. Staphylococcus aureus produces siderophores, of which the structures of staphyloferrin A and staphyloferrin B are known. Recently, the NIS biosynthetic pathway for staphyloferrin A was characterized. Here we show that, in S. aureus, the previously identified sbn (siderophore biosynthesis) locus encodes enzymes required for the synthesis of staphyloferrin B, an alpha-hydroxycarboxylate siderophore comprised of l-2,3-diaminopropionic acid, citric acid, 1,2-diaminoethane and alpha-ketoglutaric acid. Staphyloferrin B NIS biosynthesis was recapitulated in vitro, using purified recombinant Sbn enzymes and the component substrates. In vitro synthesized staphyloferrin B readily promoted the growth of iron-starved S. aureus, via the ABC transporter SirABC. The SbnCEF synthetases and a decarboxylase, SbnH, were necessary and sufficient to produce staphyloferrin B in reactions containing component substrates l-2,3-diaminopropionic acid, citric acid and alpha-ketoglutaric acid. Since 1,2-diaminoethane was not required, this component of the siderophore arises from the SbnH-dependent decarboxylation of a 2,3-diaminoproprionic acid-containing intermediate. Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses of a series of enzyme reactions identified mass ions corresponding to biosynthetic intermediates, allowing for the first proposed biosynthetic pathway for staphyloferrin B.
引用
收藏
页码:594 / 608
页数:15
相关论文
共 30 条
[1]   Recent advances in siderophore biosynthesis [J].
Barry, Sarah M. ;
Challis, Gregory L. .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2009, 13 (02) :205-215
[2]   Characterization of staphyloferrin A biosynthetic and transport mutants in staphylococcus aureus [J].
Beasley, Federico C. ;
Vines, Enrique D. ;
Grigg, Jason C. ;
Zheng, Qin ;
Liu, Suya ;
Lajoie, Gilles A. ;
Murphy, Michael E. P. ;
Heinrichs, David E. .
MOLECULAR MICROBIOLOGY, 2009, 72 (04) :947-963
[3]   Analysis of Achromobactin Biosynthesis by Pseudomonas syringae pv. syringae B728a [J].
Berti, Andrew D. ;
Thomas, Michael G. .
JOURNAL OF BACTERIOLOGY, 2009, 191 (14) :4594-4604
[4]   Ralstonia solanacearum iron scavenging by the siderophore staphyloferrin B is controlled by PhcA, the global virulence regulator [J].
Bhatt, G ;
Denny, TP .
JOURNAL OF BACTERIOLOGY, 2004, 186 (23) :7896-7904
[5]   A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases [J].
Challis, GL .
CHEMBIOCHEM, 2005, 6 (04) :601-611
[6]   Identification and Characterization of the Staphylococcus aureus Gene Cluster Coding for Staphyloferrin A [J].
Cotton, Jennifer L. ;
Tao, Jianshi ;
Balibar, Carl J. .
BIOCHEMISTRY, 2009, 48 (05) :1025-1035
[7]   Genetics and assembly line enzymology of siderophore biosynthesis in bacteria [J].
Crosa, JH ;
Walsh, CT .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2002, 66 (02) :223-+
[8]   Role of siderophore biosynthesis in virulence of Staphylococcus aureus:: Identification and characterization of genes involved in production of a siderophore [J].
Dale, SE ;
Doherty-Kirby, A ;
Lajoie, G ;
Heinrichs, DE .
INFECTION AND IMMUNITY, 2004, 72 (01) :29-37
[9]   Involvement of SirABC in iron-siderophore import in Staphylococcus aureus [J].
Dale, SE ;
Sebulsky, MT ;
Heinrichs, DE .
JOURNAL OF BACTERIOLOGY, 2004, 186 (24) :8356-8362
[10]   CHARACTERIZATION OF IUCA AND IUCC GENES OF THE AEROBACTIN SYSTEM OF PLASMID COLV-K30 IN ESCHERICHIA-COLI [J].
DELORENZO, V ;
NEILANDS, JB .
JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :350-355