The nonenzymatic hydrolysis of oligoribonucleotides VII. Structural elements affecting hydrolysis.

被引：27

作者：

Bibillo, A ^{[1
]}

Figlerowicz, M ^{[1
]}

Ziomek, K ^{[1
]}

Kierzek, R ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2000年 / 19卷 / 5-6期

关键词：

D O I：

10.1080/15257770008033037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several elements of oligoribonucleotide structure are important for efficient hydrolysis. We have found that the following factors influence oligoribonucleotide hydrolysis: (i) single-stranded structure of RNA flanking the scissile phosphodiester bond, (ii) the substituent on atom C-5 of the uridine adjacent to the cleaved internucleotide bond, (iii) the position of the scissile UA phosphodiester bond within a hairpin loop, (iv) the concentration of formamide, urea, ethanol and sodium chloride.

引用

页码：977 / 994

页数：18

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