Characterization of Mycobacterium tuberculosis LexA: Recognition of a Cheo (Bacillus-type SOS) box

被引：41

作者：

Movahedzadeh, F ^{[1
]}

Colston, MJ ^{[1
]}

Davis, EO ^{[1
]}

机构：

[1] NATL INST MED RES,DIV MYCOBACTERIAL RES,LONDON NW7 1AA,ENGLAND

来源：

MICROBIOLOGY-UK | 1997年 / 143卷

关键词：

Mycobacterium tuberculosis; LexA; RecA; DNA-damage-inducible genes; DNA-binding protein;

D O I：

10.1099/00221287-143-3-929

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene coding for the Mycobacterium tuberculosis homologue of LexA has been cloned and sequenced. Amino acids required for autocatalytic cleavage are conserved, whereas those important for specific DNA binding are not, when compared with Escherichia coli LexA. The transcriptional start site was mapped and a DNA sequence motif was identified which resembled the consensus Cheo box sequence involved in the regulation of DNA-damage-inducible genes in Bacillus subtilis. The M. tuberculosis LexA protein was overexpressed in E. coli and purified by means of a His tag. The purifed LexA was shown to bind to the Cheo box sequence found upstream of its own gene.

引用

页码：929 / 936

页数：8

共 36 条

[1] MECHANISM OF ACTION OF THE LEXA GENE-PRODUCT [J].

BRENT, R ;

PTASHNE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (07) :4204-4208

[2] CLONING AND CHARACTERIZATION OF DNA DAMAGE-INDUCIBLE PROMOTER REGIONS FROM BACILLUS-SUBTILIS [J].

CHEO, DL ;

BAYLES, KW ;

YASBIN, RE .

JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1696-1703

[3] EVIDENCE OF SELECTION FOR PROTEIN INTRONS IN THE RECAS OF PATHOGENIC MYCOBACTERIA [J].

DAVIS, EO ;

THANGARAJ, HS ;

BROOKS, PC ;

COLSTON, MJ .

EMBO JOURNAL, 1994, 13 (03) :699-703

[4] NOVEL STRUCTURE OF THE RECA LOCUS OF MYCOBACTERIUM-TUBERCULOSIS IMPLIES PROCESSING OF THE GENE-PRODUCT [J].

DAVIS, EO ;

SEDGWICK, SG ;

COLSTON, MJ .

JOURNAL OF BACTERIOLOGY, 1991, 173 (18) :5653-5662

[5] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[6] SOLUTION STRUCTURE OF THE LEXA REPRESSOR DNA-BINDING DOMAIN DETERMINED BY H-1-NMR SPECTROSCOPY [J].

FOGH, RH ;

OTTLEBEN, G ;

RUTERJANS, H ;

SCHNARR, M ;

BOELENS, R ;

KAPTEIN, R .

EMBO JOURNAL, 1994, 13 (17) :3936-3944

[7] NUCLEOTIDE-SEQUENCE ANALYSIS AND COMPARISON OF THE LEXA GENES FROM SALMONELLA-TYPHIMURIUM, ERWINIA-CAROTOVORA, PSEUDOMONAS-AERUGINOSA AND PSEUDOMONAS-PUTIDA [J].

GARRIGA, X ;

CALERO, S ;

BARBE, J .

MOLECULAR AND GENERAL GENETICS, 1992, 236 (01) :125-134

[8] The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: Comparison of promoter elements and of neighbouring upstream genes [J].

GonzalezyMerchand, JA ;

Colston, MJ ;

Cox, RA .

MICROBIOLOGY-UK, 1996, 142 :667-674

[9] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[10] NUCLEOTIDE-SEQUENCE OF THE LEXA GENE OF ESCHERICHIA-COLI [J].

HORII, T ;

OGAWA, T ;

OGAWA, H .

CELL, 1981, 23 (03) :689-697

← 1 2 3 4 →