A new class of tetraspanins in fungi

被引:34
作者
Gourgues, M
Clergeot, PH
Veneault, C
Cots, J
Sibuet, S
Brunet-Simon, A
Levis, C
Langin, T
Lebrun, MH
机构
[1] CNRS, Bayer Cropsci, UMR1932, F-69009 Lyon, France
[2] Inst Biotechnol Plantes, Orsay, France
[3] INRA, F-78026 Versailles, France
关键词
tetraspanin; PLS1; Magnaporthe grisea; Botrytis cinerea; Colletotrichum lindemuthianum; Neurospora crassa;
D O I
10.1016/S0006-291X(02)02355-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tetraspanins are animal proteins involved in membrane complexes that control cell adhesion, differentiation, and motility. The PLS1 gene from rice blast fungus Magnaporthe grisea encodes a protein (Pls1p) structurally related to tetraspanins that is required for pathogenicity. In Botrytis cinerea public sequences, we identified an EST homologous to PLS1. Using degenerated oligonucleotides, we amplified sequences homologous to PLS1 in fungi Colletotrichum lindemuthianum and Neurospora crassa. Analysis of N. crassa and M. grisea genome sequences revealed the presence of a single tetraspanin gene. Thus, fungi differ from animals, which contain between 20 and 37 paralogous tetraspanin genes. Fungal proteins encoded by BcPLS1, ClPLS1, and NcPLS1 display all the structural hallmarks of tetraspanins (predicted topology with four transmembrane domains, extra- and intracellular loops; conserved cysteine-based patterns in second extracellular loop). Phylogenetic analysis suggests that these genes define a new family of orthologous genes encoding fungal-specific tetraspanins. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:1197 / 1204
页数:8
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