Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography

被引：142

作者：

Crozier, A

Jensen, E

Lean, MEJ

McDonald, MS

机构：

[1] UNIV TROMSO,DEPT PHARMACOL,N-9037 TROMSO,NORWAY

[2] UNIV GLASGOW,ROYAL INFIRM,DEPT HUMAN NUTR,GLASGOW G31 2ER,LANARK,SCOTLAND

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 761卷 / 1-2期

关键词：

plant extracts; flavonoids;

D O I：

10.1016/S0021-9673(96)00826-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Large variations were found in the effectiveness of five reversed-phase high-performance liquid chromatography columns used for the analysis of free and conjugated flavonoids. The best results were obtained with C-18 Nova-Pak, C-18 Symmetry and C-18 Genesis columns but substantial band broadening and peak asymmetry were observed when free flavonoids were chromatographed on ODS-Hypersil and LiChrospher RP-18 supports. The Symmetry and Genesis columns provided gradient elution separations of rutin, quercetin-3-glucoside, quercitrin, myricetin, luteolin, quercetin, apigenin, kaempferol and isorhamnetin. This procedure was used for the quantitative analysis of endogenous flavones and flavonols in acid hydrolysed extracts from onions and celery.

引用

页码：315 / 321

页数：7

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