Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR

被引:69
作者
Hoffmann, Bernd [1 ]
Harder, Timm [1 ]
Starick, Elke [1 ]
Depner, Klaus [1 ]
Werner, Ortrud [1 ]
Beer, Martin [1 ]
机构
[1] Friedrich Loeffler Inst Insel Riems, Inst Diagnost Virol, D-17493 Riems, Germany
关键词
D O I
10.1128/JCM.01681-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.
引用
收藏
页码:600 / 603
页数:4
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