Spectrophotometric determination of fluoxetine by batch and flow injection methods

被引：20

作者：

Afkhami, Abbas ^{[1
]}

Madrakian, Tayyebeh ^{[1
]}

Khalafi, Lida ^{[1
]}

机构：

[1] Bu Ali Sina Univ, Fac Chem, Hamadan 65174, Iran

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2006年 / 54卷 / 12期

关键词：

competitive complexation; fluoxetine determination; inclusion complex; beta-cyclodextrin; flow injection analysis (FIA); pharmaceuticals; UV/VIS;

D O I：

10.1248/cpb.54.1642

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A rapid, simple, and accurate spectrophotometric method is presented for the determination of fluoxetine by batch and flow injection analysis methods. The method is based on fluoxetine competitive complexation reaction with phenolphthalein-beta-cyclodextrin (PHP-beta-CD) inclusion complex. The increase in the absorbance of the solution at 554 urn by the addition of fluoxetine was measured. The formation constant for fluoxetin-beta-CD was calculated by non-linear least squares fitting. Fluoxetine can be determined in the range 7.0x10(-6)-2.4 x 10(-4) mol l(-1) and 5.0x10(-5)-1.0x10(-2) mol l(-1) by batch and flow methods, respectively. The limit of detection and limit of quantification were respectively 4.13x10(-6) mol l(-1) and 1.38 x 10(-5) mol l(-1) for batch and 2.46 x 10(-5) mol l(-1) and 8.22 x 10(-5) mol l(-1) for flow method. The sampling rate in flow injection analysis method was 80 +/- 5 samples h(-1). The method was applied to the determination of fluoxetine in pharmaceutical formulations and after addition to human urine samples.

引用

页码：1642 / 1646

页数：5

共 26 条

[1] Spectrophotometric determination of conditional acidity constant as a function of β-cyclodextrin concentration for some organic acids using rank annihilation factor analysis [J].