Oxidative enzymes possess catalytic activity in systems with ionic liquids

被引：116

作者：

Hinckley, G ^{[1
]}

Mozhaev, VV ^{[1
]}

Budde, C ^{[1
]}

Khmelnitsky, YL ^{[1
]}

机构：

[1] Albany Mol Res Inc, Biosci Div, Mt Prospect, IL 60056 USA

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 24期

关键词：

enzyme catalysis; ionic liquids; laccase; mediators; peroxidase;

D O I：

10.1023/A:1021305229969

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oxidative enzymes, laccase C from Trametes sp. and horseradish and soybean peroxidases, catalyzed oxidation reactions in systems with ionic liquids whose content varied from several volume percent to almost total non-aqueous ionic liquids. Similar to the effects produced by standard organic solvents used in non-aqueous enzymology, catalytic activity of the enzymes was decreased by adding a water-miscible ionic liquid, 4-methyl-N-butylpyridinium tetrafluoroborate, or by suspending the enzyme in a water-immiscible ionic liquid, 1-butyl-3-methylimdizaolium hexafluorophosphate. For the oxidation of anthracene, catalyzed by laccase C and assisted by a number of mediators, addition of 4-methyl-N-butylpyridinium tetrafluoroborate, instead of tert-butanol, increased the yield of the oxidation product several-fold.

引用

页码：2083 / 2087

页数：5

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