α-Lactalbumin precipitation from commercial whey protein concentrates

被引:29
作者
Lucena, M. Eugenia
Alvarez, Silvia
Menendez, Carlos
Riera, Francisco A.
Alvarez, Ricardo
机构
[1] Univ Oviedo, Dept Chem Engn & Environm Technol, E-33071 Oviedo, Spain
[2] Univ Politecn Valencia, Dept Chem & Nucl Engn, Valencia 46022, Spain
关键词
whey protein concentrates (WPCs); alpha-lactalbumin; protein precipitation;
D O I
10.1016/j.seppur.2006.05.024
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The precipitation of alpha-lactalbumin (alpha-La) by acidification of whey and whey protein concentrates (WPCs) was studied in this work. Three different acids (HCl, citric acid and lactic acid) were considered to perform the precipitation step. Sweet whey, WPCs with different protein concentration and whey protein isolates (WPIs) were used as feed solutions. The two organic acids were able to complexate the Ca2+ ions. However, when hydrochloric acid was used, protein precipitation was due to the irreversible denaturation of the proteins. The precipitation process was performed at different operating conditions (protein concentration, pH, temperature and acid/Ca2+ molar ratio) at laboratory scale in order to be optimised. The optimum initial protein concentration was observed to be around 12 g/L. A temperature of 50 degrees C, a pH value of 4.0, and an organic acid/Ca2+ molar ratio higher than 9 were the optimum values to perform the precipitation at laboratory scale. When the precipitation process was carried out at a pH value close to the isoelectric point (IP) of alpha-La and it was combined with calcium ion complexation, alpha-La was observed to precipitate together with BSA and immunoglobulins. However, beta-lactoglobulin (beta-Lg) remained in solution due to the stabilisation of this protein at low Ca2+ concentrations. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:446 / 453
页数:8
相关论文
共 32 条
[1]  
Bottomley R. C., 1991, US Patent, Patent No. [5008376, 5,008,376]
[2]  
Bramaud C, 1997, LAIT, V77, P411, DOI 10.1051/lait:1997328
[3]   THERMAL ISOELECTRIC PRECIPITATION OF ALPHA-LACTALBUMIN FROM A WHEY-PROTEIN CONCENTRATE - INFLUENCE OF PROTEIN-CALCIUM COMPLEXATION [J].
BRAMAUD, C ;
AIMAR, P ;
DAUFIN, G .
BIOTECHNOLOGY AND BIOENGINEERING, 1995, 47 (02) :121-130
[4]   Membrane fractionation of milk:: state of the art and challenges [J].
Brans, G ;
Schroën, CGPH ;
van der Sman, RGM ;
Boom, RM .
JOURNAL OF MEMBRANE SCIENCE, 2004, 243 (1-2) :263-272
[5]   Separation of α-lactalbumin and β-lactoglobulin using membrane ultrafiltration [J].
Cheang, B ;
Zydney, AL .
BIOTECHNOLOGY AND BIOENGINEERING, 2003, 83 (02) :201-209
[6]  
DAFONSECA L, 2002, Patent No. 10095856
[7]   A DIFFERENTIAL SCANNING CALORIMETRIC STUDY OF THE THERMAL-DENATURATION OF BOVINE BETA-LACTOGLOBULIN - THERMAL-BEHAVIOR AT TEMPERATURES UP TO 100-DEGREES-C [J].
DEWIT, JN ;
SWINKELS, GAM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 624 (01) :40-50
[8]   Process steps for the preparation of purified fractions of α-lactalbumin and β-lactoglobulin from whey protein concentrates [J].
Gésan-Guiziou, G ;
Daufin, G ;
Timmer, M ;
Allersma, D ;
Van der Horst, C .
JOURNAL OF DAIRY RESEARCH, 1999, 66 (02) :225-236
[9]   THERMAL-STABILITY OF FATTY ACID SERUM ALBUMIN COMPLEXES STUDIED BY DIFFERENTIAL SCANNING CALORIMETRY [J].
GUMPEN, S ;
HEGG, PO ;
MARTENS, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 574 (02) :189-196
[10]   Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (Review) [J].
Ha, E ;
Zemel, MB .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2003, 14 (05) :251-258